徐州医学院学报
徐州醫學院學報
서주의학원학보
ACTA ACADEMIAE MEDICINAE XUZHOU
2014年
4期
264-267
,共4页
多不饱和脂肪酸%子宫内膜癌%皮下种植瘤%AKT%mTOR
多不飽和脂肪痠%子宮內膜癌%皮下種植瘤%AKT%mTOR
다불포화지방산%자궁내막암%피하충식류%AKT%mTOR
polyunsaturated fatty acids%endometrial carcinoma%subcutaneously transplantable tumor%AKT%mTOR
目的：研究不同比例n-6/n-3多不饱和脂肪酸（ PUFAs）对人子宫内膜癌瘤组织中AKT、mTOR表达的影响。方法建立人子宫内膜癌小鼠皮下种植瘤模型，40只实验BALB/C模型小鼠随机分为A组（ n-6 PU-FAs）、B组（10∶1 n-6/n-3 PUFAs）、C组（对照）、D组（1∶1 n-6/n-3 PUFAs）、E组（n-3 PUFAs），每组8只。A、B、D、E组分别灌胃给予不同比例 PUFAs，C组给予等量生理盐水。6周后处死小鼠，切取皮下种植瘤组织，苏木精-伊红染色镜下定位观察肿瘤组织形态，免疫组化SP法检测种植瘤组织中AKT、mTOR蛋白表达， RT-PCR法检测各组种植瘤组织中AKT、mTOR的mRNA表达。结果苏木精-伊红染色结果确认皮下种植瘤模型建立成功；免疫组化SP法和RT-PCR结果显示n-6 PUFAs和10∶1 n-6/n-3 PuFAs 组中AKT、mTOR的蛋白和mRNA的表达增加，与对照组相比差异有统计学意义（ P＜0．05）；n-3 PUFAs和1∶1 n-6/n-3 PUFAs 组中的中AKT、mTOR的蛋白和mRNA表达下降，与对照组相比差异有统计学意义（ P＜0．05）。结论不同比例的 n-6/n-3 PUFAs对人子宫内膜癌的作用机制可能是通过PI3k/AKT/mTOR信号通路调节 AKT、mTOR的表达而起作用的。
目的：研究不同比例n-6/n-3多不飽和脂肪痠（ PUFAs）對人子宮內膜癌瘤組織中AKT、mTOR錶達的影響。方法建立人子宮內膜癌小鼠皮下種植瘤模型，40隻實驗BALB/C模型小鼠隨機分為A組（ n-6 PU-FAs）、B組（10∶1 n-6/n-3 PUFAs）、C組（對照）、D組（1∶1 n-6/n-3 PUFAs）、E組（n-3 PUFAs），每組8隻。A、B、D、E組分彆灌胃給予不同比例 PUFAs，C組給予等量生理鹽水。6週後處死小鼠，切取皮下種植瘤組織，囌木精-伊紅染色鏡下定位觀察腫瘤組織形態，免疫組化SP法檢測種植瘤組織中AKT、mTOR蛋白錶達， RT-PCR法檢測各組種植瘤組織中AKT、mTOR的mRNA錶達。結果囌木精-伊紅染色結果確認皮下種植瘤模型建立成功；免疫組化SP法和RT-PCR結果顯示n-6 PUFAs和10∶1 n-6/n-3 PuFAs 組中AKT、mTOR的蛋白和mRNA的錶達增加，與對照組相比差異有統計學意義（ P＜0．05）；n-3 PUFAs和1∶1 n-6/n-3 PUFAs 組中的中AKT、mTOR的蛋白和mRNA錶達下降，與對照組相比差異有統計學意義（ P＜0．05）。結論不同比例的 n-6/n-3 PUFAs對人子宮內膜癌的作用機製可能是通過PI3k/AKT/mTOR信號通路調節 AKT、mTOR的錶達而起作用的。
목적：연구불동비례n-6/n-3다불포화지방산（ PUFAs）대인자궁내막암류조직중AKT、mTOR표체적영향。방법건립인자궁내막암소서피하충식류모형，40지실험BALB/C모형소서수궤분위A조（ n-6 PU-FAs）、B조（10∶1 n-6/n-3 PUFAs）、C조（대조）、D조（1∶1 n-6/n-3 PUFAs）、E조（n-3 PUFAs），매조8지。A、B、D、E조분별관위급여불동비례 PUFAs，C조급여등량생리염수。6주후처사소서，절취피하충식류조직，소목정-이홍염색경하정위관찰종류조직형태，면역조화SP법검측충식류조직중AKT、mTOR단백표체， RT-PCR법검측각조충식류조직중AKT、mTOR적mRNA표체。결과소목정-이홍염색결과학인피하충식류모형건립성공；면역조화SP법화RT-PCR결과현시n-6 PUFAs화10∶1 n-6/n-3 PuFAs 조중AKT、mTOR적단백화mRNA적표체증가，여대조조상비차이유통계학의의（ P＜0．05）；n-3 PUFAs화1∶1 n-6/n-3 PUFAs 조중적중AKT、mTOR적단백화mRNA표체하강，여대조조상비차이유통계학의의（ P＜0．05）。결론불동비례적 n-6/n-3 PUFAs대인자궁내막암적작용궤제가능시통과PI3k/AKT/mTOR신호통로조절 AKT、mTOR적표체이기작용적。
Objective To investigate the effects of different ratios of n -6/n-3 polyunsaturated fatty acids on the expressions of AKT and mTOR in mice with endometrial carcinoma .Methods A tumor model of mice was established where the mice were subcutaneously injected with human endometrial carcinoma .The mice ( n=40 ) were randomly di-vided into five groups according to their different treatments:group A (n-6 PUFAs), group B( 10∶1 n-6/n-3 PU-FAs), group C (control group), group D (1∶1 n-6/n-3 PUFAs) and group E (n-3 PUFAs), eight mice in each group.The mice in experimental groups were intragastrically administrated with different proportional PUFAs ,while the mice in control group were fed with an equal amount of normal saline .Six weeks later , these mice were sacrificed and the subcutaneous tumor tissues were excised .The histomorphological changes of the tissues were detected by hematoxylin -e-osin staining .The expressions of AKT and mTOR were examined by SP -IHC method , while the amounts of AKT and mTOR mRNA were accessed by RT -PCR.Results The establishment of the model of mice with human endometrial carcinoma was confirmed by HE staining .Then enhanced expressions of AKT and mTOR at protein and mRNA levels were found in the n-6 PUFAs group and the 10∶1 n-6/n-3 PUFAs group, when compared with the control group (P<0.05).However, the n-3 PUFAs group and the 1∶1 n-6/n-3 PUFAs group presented reduced protein and mRNA expressions of AKT and mTOR than the control group (P<0.05).C onclusion The effects of different ratios of n -6/n-3 PUFAs on endometrial carcinoma may be attributed to various abilities of the PUFAs to regulate the expressions of AKT and mTOR through the PI3k/AKT/mTOR signal pathway.
