CAJ | 학술논문

目的采用实时荧光PCR方法快速检测公共场所从业人员肠道致病菌，了解实时荧光PCR方法的应用价值.方法随机采集10087份从业人员肛拭子标本分别进行实时荧光PCR方法和培养法检测沙门氏菌和志贺氏菌，分析实时荧光PCR方法的检出率.结果10087份标本中，实时荧光PCR方法共检测出两种肠道菌16例，其中沙门氏菌11例，志贺氏菌5例；阳性率分别为1.09‰和0.5‰.而培养法检测出沙门菌为9例，志贺菌为4例，阳性率分别为0.89‰和0.4‰；另外男性两个致病菌阳性检出率比女性阳性检出率低.结论实时荧光PCR方法可以很好的应用在从业人员肠道致病菌的筛查，该方法可以节省劳动力，降低工作人员的工作强度.并且可以节省从业人员拿到健康证时间.
목적채용실시형광PCR방법쾌속검측공공장소종업인원장도치병균，료해실시형광PCR방법적응용개치.방법수궤채집10087빈종업인원항식자표본분별진행실시형광PCR방법화배양법검측사문씨균화지하씨균，분석실시형광PCR방법적검출솔.결과10087빈표본중，실시형광PCR방법공검측출량충장도균16례，기중사문씨균11례，지하씨균5례；양성솔분별위1.09‰화0.5‰.이배양법검측출사문균위9례，지하균위4례，양성솔분별위0.89‰화0.4‰；령외남성량개치병균양성검출솔비녀성양성검출솔저.결론실시형광PCR방법가이흔호적응용재종업인원장도치병균적사사，해방법가이절성노동력，강저공작인원적공작강도.병차가이절성종업인원나도건강증시간.
Objective:To evaluate the application of real-time PCR method for rapid detection of Salmonella and Shigella form swabs of practitioners in food industry and public places. Methods:Random collect of 10087 swabs samples from practitioners.Respectively, detected of Salmonella and Shigella with real-time PCR and plate culture methods, analysised the detection rate of real-time PCR method. Results:With real-time PCR method for Salmonella and Shigella, 11 swabs and 5 swabs were positive, with positive rate being 1.09‰ and 0.5‰, respectively.With plate culture method for Salmonella and Shigella, 9 swabs and 4 swabs were positive, with positive rate being 0.89‰ and 0.4‰, respectively. The positive detection rates of Salmonella and Shigella, males than in females.Conclusion: Application of real-time PCR method can save labor and reduce work load, and can save time for getting health reports.