中外健康文摘
中外健康文摘
중외건강문적
WORLD HEALTH DIGEST
2012年
51期
48-48
,共1页
人肺腺癌细胞系A2%移植瘤
人肺腺癌細胞繫A2%移植瘤
인폐선암세포계A2%이식류
A2 cell line%implantation tumor
目的探讨SCID小鼠人肺腺癌移植瘤模型的建立及其生物学特性。方法将人肺腺癌细胞系A2细胞移植于SCID小鼠皮下，用其瘤块传代至转移率为95%以上，共6代。采用光镜、MTT等手段对各代移植瘤的形态特征、移植瘤的增殖、侵袭和转移等生物学特性进行研究。结果移植瘤的形态学特点与原始细胞系完全一致，而原代与晚代移植瘤之间的比较，显示进一步恶化的特征。
目的探討SCID小鼠人肺腺癌移植瘤模型的建立及其生物學特性。方法將人肺腺癌細胞繫A2細胞移植于SCID小鼠皮下，用其瘤塊傳代至轉移率為95%以上，共6代。採用光鏡、MTT等手段對各代移植瘤的形態特徵、移植瘤的增殖、侵襲和轉移等生物學特性進行研究。結果移植瘤的形態學特點與原始細胞繫完全一緻，而原代與晚代移植瘤之間的比較，顯示進一步噁化的特徵。
목적탐토SCID소서인폐선암이식류모형적건립급기생물학특성。방법장인폐선암세포계A2세포이식우SCID소서피하，용기류괴전대지전이솔위95%이상，공6대。채용광경、MTT등수단대각대이식류적형태특정、이식류적증식、침습화전이등생물학특성진행연구。결과이식류적형태학특점여원시세포계완전일치，이원대여만대이식류지간적비교，현시진일보악화적특정。
Objective: To establish a SCID mouse model of human lung adenocarcinoma and study its biological behavoir. Methods: A2 cells were inoculated in SCID mouse,and after successfully tumor formation ,the generated tumors were implanted into . MTT was used to detect the growth of A2 cells.Results: The success rate of implantion was 95%. Conclusion: The SCID mouse model of human lung adenocarcinoma of A2 cell line ,with ideal implantation and growth rate ,allows convenient observation.
