CAJ | 학술논문

借助 HPLC-UV-MS 方法对云南洋蓟中多酚类化合物进行分析鉴定和含量测定.液相色谱分离采用ZORBAX Eclipse XDB-C18分析柱，以乙腈和水（含0.08%甲酸）为流动相梯度洗脱，流速为0.5 mL/min.根据多级质谱分析，并与对照品和文献数据比较，确定洋蓟样品甲醇提取液中含有19种多酚类化合物.色谱实验结果表明绿原酸、木犀草苷和水飞蓟宾在其线性范围内具有良好的线性关系（R2≥0.9998），最低检测限（LOD）分别为0.153μg/mL、0.336μg/mL 和0.313μg/mL，且在洋蓟样品中的含量分别为27.79 mg/g、10.25 mg/g和58.72 mg/g.
차조 HPLC-UV-MS 방법대운남양계중다분류화합물진행분석감정화함량측정.액상색보분리채용ZORBAX Eclipse XDB-C18분석주，이을정화수（함0.08%갑산）위류동상제도세탈，류속위0.5 mL/min.근거다급질보분석，병여대조품화문헌수거비교，학정양계양품갑순제취액중함유19충다분류화합물.색보실험결과표명록원산、목서초감화수비계빈재기선성범위내구유량호적선성관계（R2≥0.9998），최저검측한（LOD）분별위0.153μg/mL、0.336μg/mL 화0.313μg/mL，차재양계양품중적함량분별위27.79 mg/g、10.25 mg/g화58.72 mg/g.
In this paper, we conducted an analysis identification and content determination of polyphenolic compounds in artichoke (Cynara scolymus L.) from Yunnan by the method of HPLC-UV-MS. The liquid chromatographic separation was performed by the ZORBAX Eclipse XDB-C18 analytical column, which based in the mobile phase gradient elution with acetonitrile and water (containing 0.08%formic acid) at a flow rate of 0.5 mL/min. According to the analysis of multistage mass spectrometry, and the comparison with reference compounds and literature data, we confirmed that methanol extract in artichoke samples contained 19 kinds of polyphenolic compounds. Chromatographic experimental results showed that chlorogenic acid, luteoloside and silibinin had a good linear regression (R2≥0.9998) in its linear ranges, and the limits of detection (LODs) were 0.153μg/mL、0.336μg/mL and 0.313μg/mL, respectively. And the content in artichokes sample were 27.79 mg/g, 10.25 mg/g and 58.72 mg/g, respectively.