湖北大学学报（自然科学版）
湖北大學學報（自然科學版）
호북대학학보（자연과학판）
2013年
3期
288-293
,共6页
万梦雪%王敏%周馥荔%杨兰芳
萬夢雪%王敏%週馥荔%楊蘭芳
만몽설%왕민%주복려%양란방
砷%绿豆%黑豆%种子萌发%半抑制浓度
砷%綠豆%黑豆%種子萌髮%半抑製濃度
신%록두%흑두%충자맹발%반억제농도
arsenic%mung bean(Vigna radiata)%black soybean(Glycine max var)%seed germination%half maximal inhibitory concentration
为了认识砷污染与种子萌发的关系，进行绿豆和黑豆加砷砂培种子萌发实验．结果表明：低浓度的砷（1 mg kg -1）对绿豆和黑豆种子萌发有促进作用，对萌发质量的促进作用可达12％以上，当砷水平超过一定浓度（5 mg kg -1）则表现出明显的抑制作用，且随砷水平增加，抑制作用增强．砷处理第6天绿豆的发芽率和萌发质量的最大抑制率分别为88．2％和56．2％，黑豆发芽率和萌发质量的最大抑制率分别为57．9％和43．6％．同一砷水平下，砷对绿豆和黑豆萌发的抑制作用均随萌发时间的延长而减弱．在高砷水平15和20 mg kg -1砂下，黑豆发芽率高于绿豆，砷对黑豆发芽率的抑制率小于绿豆．无论是发芽率还是萌发质量，黑豆的半抑制浓度均大于绿豆．总之，低剂量的砷可以促进绿豆和黑豆种子萌发，大于5 mg kg -1的砷则抑制种子萌发，砷毒害导致种子发芽率和萌发质量降低，萌发延迟，豆芽变短；黑豆种子的耐高水平砷毒害的能力比绿豆种子强．
為瞭認識砷汙染與種子萌髮的關繫，進行綠豆和黑豆加砷砂培種子萌髮實驗．結果錶明：低濃度的砷（1 mg kg -1）對綠豆和黑豆種子萌髮有促進作用，對萌髮質量的促進作用可達12％以上，噹砷水平超過一定濃度（5 mg kg -1）則錶現齣明顯的抑製作用，且隨砷水平增加，抑製作用增彊．砷處理第6天綠豆的髮芽率和萌髮質量的最大抑製率分彆為88．2％和56．2％，黑豆髮芽率和萌髮質量的最大抑製率分彆為57．9％和43．6％．同一砷水平下，砷對綠豆和黑豆萌髮的抑製作用均隨萌髮時間的延長而減弱．在高砷水平15和20 mg kg -1砂下，黑豆髮芽率高于綠豆，砷對黑豆髮芽率的抑製率小于綠豆．無論是髮芽率還是萌髮質量，黑豆的半抑製濃度均大于綠豆．總之，低劑量的砷可以促進綠豆和黑豆種子萌髮，大于5 mg kg -1的砷則抑製種子萌髮，砷毒害導緻種子髮芽率和萌髮質量降低，萌髮延遲，豆芽變短；黑豆種子的耐高水平砷毒害的能力比綠豆種子彊．
위료인식신오염여충자맹발적관계，진행록두화흑두가신사배충자맹발실험．결과표명：저농도적신（1 mg kg -1）대록두화흑두충자맹발유촉진작용，대맹발질량적촉진작용가체12％이상，당신수평초과일정농도（5 mg kg -1）칙표현출명현적억제작용，차수신수평증가，억제작용증강．신처리제6천록두적발아솔화맹발질량적최대억제솔분별위88．2％화56．2％，흑두발아솔화맹발질량적최대억제솔분별위57．9％화43．6％．동일신수평하，신대록두화흑두맹발적억제작용균수맹발시간적연장이감약．재고신수평15화20 mg kg -1사하，흑두발아솔고우록두，신대흑두발아솔적억제솔소우록두．무론시발아솔환시맹발질량，흑두적반억제농도균대우록두．총지，저제량적신가이촉진록두화흑두충자맹발，대우5 mg kg -1적신칙억제충자맹발，신독해도치충자발아솔화맹발질량강저，맹발연지，두아변단；흑두충자적내고수평신독해적능력비록두충자강．
Arsenic is a toxic element both to animals and plants ,and arsenic pollution may reduce the crop yields and threat to food safety .An experiment germinating mung bean and black soybean seeds by arsenic addition in sands was conducted to understand the effect of arsenic on seed germination ,in which the situation of seed germination was observed and the percentage of seed germination and germination weight was determined . The results showed that 1 mg kg-1 arsenic addition stimulated the mung bean and black soybean seed germination and increased about 12% of the germination weight ,but the seed germination was significantly suppressed when the arsenic addition was over 5 mg kg-1 , and thereafter the suppression to seed germination was increased with the increasing of arsenic concentrations .The highest decreasing rate of percentage of seed germination and the germination weight at 6 days after incubation was 88 .2% and 56 .2% for mung bean and 57 .9%and 43 .6% for black soybean ,respectively .The suppression of arsenic to seed germination of mung bean and black soybean in the same arsenic level decreased with incubation time .Under the same high arsenic concentration ,such as 15 and 20 mg kg -1 ,the seed germination percentage of black soybean was higher than that of mung bean ,while the suppression rate to the percentage of seed germination of black soybean was lower than that of mung bean .Both germination rate and germination weight , the half maximal inhibitory concentration of black soybean seeds was higher than that of mung bean seeds .It was concluded that the seed germination of mung bean and black soybean was promoted by low arsenic addition and suppressed by over 5 mg kg-1 arsenic addition and the arsenic toxicity to seed germination was the declination of percentage of seed germination and germination weight , germination retardation and the short buds , but the arsenic tolerance of black soybean seeds was stronger than that of mung bean seeds .
