CAJ | 학술논문

白腐真菌在低阶煤炭深加工方面有很大的应用潜力。为依据低阶煤降解机理，报道以喹啉作为低阶煤含氮模型化合物，利用白腐真菌在DOX培养基中对其进行微生物降解，通过测定降解体系中木质素过氧化物酶（LiP）、锰过氧化物酶（MnP）、漆酶（Lac）和多酚氧化酶（PPO）的活性，并利用红外光谱仪、气相色谱-质谱分析仪分析了降解产物。结果发现，喹啉在Lac、LiP和PPO等共同催化下发生了羟基化、醌基化、开环、C-N断裂、氧化等作用，得到对应的8种主要降解产物，从而推测出白腐真菌对喹啉的降解历程。
백부진균재저계매탄심가공방면유흔대적응용잠력。위의거저계매강해궤리，보도이규람작위저계매함담모형화합물，이용백부진균재DOX배양기중대기진행미생물강해，통과측정강해체계중목질소과양화물매（LiP）、맹과양화물매（MnP）、칠매（Lac）화다분양화매（PPO）적활성，병이용홍외광보의、기상색보-질보분석의분석료강해산물。결과발현，규람재Lac、LiP화PPO등공동최화하발생료간기화、곤기화、개배、C-N단렬、양화등작용，득도대응적8충주요강해산물，종이추측출백부진균대규람적강해역정。
White rot fungi has great potential applications in low rank coal deep processing. To study degrada-tion mechanism of low rank coal, quinoline has been used as low rank coal model compound, and degraded by white rot fungi in DOX culture. Enzymes containing lignin peroxidase (LiP), manganese peroxidase (MnP), laccase (Lac) and polyphenol oxidase (PPO) were detected in degradation systems, and degraded products were detected by FT-IR and GC-MS analyzer. Results show that Lac, LiP and PPO catalyze quino-line occur hydroxylation, quinonylation, ring opening, C-Nbreakage and oxidation reactions to receive 8 cor-responding degraded products. Thus, degradation mechanism of quinoline by white rot fungi was speculated.