CAJ | 학술논문

目的：比较各种不同类型的乳腺癌细胞系miR-34a的表达，探索作为抑癌基因的miR-34a在抑制肿瘤转移中的临床意义。方法：用micro RNA特异性试剂盒提取总RNA，应用特异性茎-环反转录实时PCR技术检测miR-34a在各种乳腺癌细胞中的表达，并进行比较分析。结果：提取的RNA质量完好，miR-34a在所检测的高侵袭性乳腺癌细胞MDA-MB-231, MDA-MB-435和BT-549中呈低表达；而在低侵袭性细胞MCF7和T47D中呈相对高表达。结论：建立了特异性茎-环反转录实时PCR检测非编码小RNA分子技术；miR-34a的表达与肿瘤细胞高侵袭性可能呈负相关，提示miR-34a作为抑癌基因可能在抑制肿瘤转移中起重要作用,在乳腺癌诊断、预防和治疗中具有重要的潜在价值。
목적：비교각충불동류형적유선암세포계miR-34a적표체，탐색작위억암기인적miR-34a재억제종류전이중적림상의의。방법：용micro RNA특이성시제합제취총RNA，응용특이성경-배반전록실시PCR기술검측miR-34a재각충유선암세포중적표체，병진행비교분석。결과：제취적RNA질량완호，miR-34a재소검측적고침습성유선암세포MDA-MB-231, MDA-MB-435화BT-549중정저표체；이재저침습성세포MCF7화T47D중정상대고표체。결론：건립료특이성경-배반전록실시PCR검측비편마소RNA분자기술；miR-34a적표체여종류세포고침습성가능정부상관，제시miR-34a작위억암기인가능재억제종류전이중기중요작용,재유선암진단、예방화치료중구유중요적잠재개치。
Objective:To compare the miR-34a expression ifles in different types of breast cancer cell lines, and to explore the clinical signiifcance for suppressing tumor metastasis. Methods:The total RNAs, including small non-coding RNA were extracted by using miRNeasy mini kit and speciifc stem-loop reverse transcription real-time PCR was performed and analyzed for detecting miR-34a expression in cell lines of breast cancer. Results:The high quality total RNAs were attained. The expression level of miR-34a was low in breast tumor cells, MDA-MB-231, MDA-MB-435, and BT-549, with high aggressiveness, whereas the expression level of miR-34a was high in breast tumor <br> cells, MCF7 and T47D, with high aggressiveness. Conclusion:We successfully establish specific stem-loop reverse transcription real-time PCR for detecting small non-coding RNA. The expression level of miR-34a may be negative correlated with tumor cells’ high aggressiveness, suggesting that as a tumor suppressor, miR-34a plays an important role in suppressing tumor metastasis, and has great potential for breast cancer diagnosis, prevention, and therapy.