中国实用医药
中國實用醫藥
중국실용의약
CHINA PRACTICAL MEDICAL
2014年
13期
43-44
,共2页
曹智刚%袁守军%陈惠鹏%徐兰平%田增月
曹智剛%袁守軍%陳惠鵬%徐蘭平%田增月
조지강%원수군%진혜붕%서란평%전증월
青蒿素衍生物%肿瘤%铁
青蒿素衍生物%腫瘤%鐵
청호소연생물%종류%철
Artemisinine derivatives%Toumer%Iron
目的:探讨青蒿素衍生物诱导肿瘤细胞产生自由基,引导肿瘤细胞死亡的机制。方法测定小鼠肿瘤动物模型的瘤体铁含量、观察青蒿素衍生物诱导肿瘤细胞产生自由基、采用单细胞彗星电泳法观察自由基对肿瘤细胞DNA杀伤的效应。结果小鼠的H22瘤体的铁含量显著高于血和肌肉、肺组织铁含量, Lewis肺癌的实验结果相同。K562细胞经青蒿素衍生物作用,能测出微量的自由基。青蒿素衍生物作用K562细胞可引起DNA损伤并溢出细胞。结论青蒿素衍生物可在含铁量较高的肿瘤细胞内产生自由基,导致肿瘤细胞死亡。
目的:探討青蒿素衍生物誘導腫瘤細胞產生自由基,引導腫瘤細胞死亡的機製。方法測定小鼠腫瘤動物模型的瘤體鐵含量、觀察青蒿素衍生物誘導腫瘤細胞產生自由基、採用單細胞彗星電泳法觀察自由基對腫瘤細胞DNA殺傷的效應。結果小鼠的H22瘤體的鐵含量顯著高于血和肌肉、肺組織鐵含量, Lewis肺癌的實驗結果相同。K562細胞經青蒿素衍生物作用,能測齣微量的自由基。青蒿素衍生物作用K562細胞可引起DNA損傷併溢齣細胞。結論青蒿素衍生物可在含鐵量較高的腫瘤細胞內產生自由基,導緻腫瘤細胞死亡。
목적:탐토청호소연생물유도종류세포산생자유기,인도종류세포사망적궤제。방법측정소서종류동물모형적류체철함량、관찰청호소연생물유도종류세포산생자유기、채용단세포혜성전영법관찰자유기대종류세포DNA살상적효응。결과소서적H22류체적철함량현저고우혈화기육、폐조직철함량, Lewis폐암적실험결과상동。K562세포경청호소연생물작용,능측출미량적자유기。청호소연생물작용K562세포가인기DNA손상병일출세포。결론청호소연생물가재함철량교고적종류세포내산생자유기,도치종류세포사망。
Objective To explore mechanism of artemisinine derivatives induced tumor of cytogenic free radical and loading to tumors cell death.Methods Iron-content for tumor body of mouse tumor of animal model was evaluated and the antipersonnel effect of tumor of cytogenic free radical for DNA with artemisinine derivatives by Mono-cell comet caraphoresis induced was studied.Results The iron-content of blood, muscle and lung is toper of tumor-body. artemisinine derivatives induced K562 tumor cell of cytogenic free radical and caused DNA damnification and flowed outward of cell envelope.Conclusion Artemisinine derivatives can induce tumor of cytogenic free radical to kill tumor.