CAJ | 학술논문

目的研究海洋生物口虾蛄乙酸乙酯提取物联合阿霉素或顺铂对人肝癌 HepG2细胞株的增殖抑制效应，并定量分析其协同、相加或拮抗的作用。 　　方法不同浓度的口虾蛄乙酸乙酯提取物联合阿霉素或顺铂处理肝癌 HepG2细胞株24 h，用 MTT 法测定细胞的生长抑制作用，并用中效原理法、金氏修正公式分析药物的联合作用。 　　结果口虾蛄乙酸乙酯提取物、阿霉素、顺铂单用或联合用药作用于肝癌 HepG2细胞株，均能抑制细胞的增殖，呈现量-效依赖性。口虾蛄乙酸乙酯提取物与传统化疗药物的联合用药增加了传统化疗药物对肝癌细胞的毒性作用，呈现出低浓度拮抗，而高浓度协同的作用。 　　结论海洋生物口虾蛄乙酸乙酯提取物作为一类新型、天然的抗肿瘤药物，可有效地抑制肝癌 HepG2细胞的增殖。
목적연구해양생물구하고을산을지제취물연합아매소혹순박대인간암 HepG2세포주적증식억제효응，병정량분석기협동、상가혹길항적작용。 　　방법불동농도적구하고을산을지제취물연합아매소혹순박처리간암 HepG2세포주24 h，용 MTT 법측정세포적생장억제작용，병용중효원리법、금씨수정공식분석약물적연합작용。 　　결과구하고을산을지제취물、아매소、순박단용혹연합용약작용우간암 HepG2세포주，균능억제세포적증식，정현량-효의뢰성。구하고을산을지제취물여전통화료약물적연합용약증가료전통화료약물대간암세포적독성작용，정현출저농도길항，이고농도협동적작용。 　　결론해양생물구하고을산을지제취물작위일류신형、천연적항종류약물，가유효지억제간암 HepG2세포적증식。
Objective To study inhibitory effects on proliferation of human hepatoma line HepG2 by ethyl acetate extract of squilla oratoria (ESO) in combination with routine anticancer chemotherapeutic agents adriamycin or cisplation, and qunatatively analyze the additive or antagonist effects between ESO and the two drugs. Methods The HepG2 cells were treated with either ESO, adriamycin or cisplatin alone, or ESO/adriamycin, ESO/cisplatin in combination for 24 h. The growth inhibition was assayed with MTT method, and the synergistic effect of different drugs was evaluated with Median-effect principle, the Revised Jin’s formula. Results The three drugs all exerted inhibitory effect on the growth of HepG2 cells in a dose-dependent manner, and the combination also decreased the proliferation of the cells. The combination with different chemotherapy agents increased cytotoxic effects on cells, and low concentration showed a synergistic effect, but the high concentrations demonstrated the antagonistic effect. Conclusions Marine squilla oratoria as a new class of natural anticancer drug, can effectively inhibit the HepG2 cells proliferation.