CAJ | 학술논문

目的观察比较正常氧和低氧环境下，不同细胞生长因子对犬骨髓间充质干细胞迁移的影响。 　　方法抽取犬骨髓，采用密度梯度离心法分离骨髓间充质干细胞并进行培养。在倒置显微镜下观察细胞的形态变化；取第3代细胞利用流式细胞术检测其表型特征；加入碱性成纤维细胞生长因子、血管内皮生长因子和胰岛素样生长因子-1，在正常氧和低氧条件下行迁移和划痕实验来观察细胞趋化能力。 　　结果细胞迁移情况与氧环境及细胞生长因子的存在和种类均有关联。碱性成纤维细胞生长因子、血管内皮生长因子和胰岛素样生长因子-1均能促进骨髓间充质干细胞的迁移，碱性成纤维细胞生长因子较其他细胞生长因子显示出更强的促进迁移作用，这种作用在低氧环境下更为明显。 　　结论低氧环境下骨髓间充质干细胞的迁移能力较正常氧环境下更强，碱性成纤维细胞生长因子、血管内皮生长因子和胰岛素样生长因子-1的存在均有利于骨髓间充质干细胞的迁移。
목적관찰비교정상양화저양배경하，불동세포생장인자대견골수간충질간세포천이적영향。 　　방법추취견골수，채용밀도제도리심법분리골수간충질간세포병진행배양。재도치현미경하관찰세포적형태변화；취제3대세포이용류식세포술검측기표형특정；가입감성성섬유세포생장인자、혈관내피생장인자화이도소양생장인자-1，재정상양화저양조건하행천이화화흔실험래관찰세포추화능력。 　　결과세포천이정황여양배경급세포생장인자적존재화충류균유관련。감성성섬유세포생장인자、혈관내피생장인자화이도소양생장인자-1균능촉진골수간충질간세포적천이，감성성섬유세포생장인자교기타세포생장인자현시출경강적촉진천이작용，저충작용재저양배경하경위명현。 　　결론저양배경하골수간충질간세포적천이능력교정상양배경하경강，감성성섬유세포생장인자、혈관내피생장인자화이도소양생장인자-1적존재균유리우골수간충질간세포적천이。
Objective This study was aimed to observe and compare the influences of cell growth factors on the migration of bone mesenchymal stem cells (BMSCs) of canines under normoxia and hypoxia. Methods BMSCs were obtained from canines’ bone marrow using density gradient centrifugation. Their growth status was observed under the inverted microscope continuously. Their phenotypic characteristics of the third generation of BMSCs were analysed through flow cytometry, and their migration ability under the normal and hypoxia environment with basic fibroblast growth factor, vascular endothelial growth factor and insulin-like growth factor-1 was measured through Boyden chamber methods and wound healing assay. Results There was relevance between the cell migration and oxygen content, the presence or absence of cell growth factors and their kinds. Basic fibroblast growth factor, vascular endothelial growth factor and insulin-like growth factor-1 can promote BMSCs to migration. Among them, basic fibroblast growth factor showed stonger promotion of the migration of BMSCs than others, especially under hypoxia environment. Conclusion The ability of migration of BMSCs is better under hypoxia environment compaired with normal oxygen environment. The existence of basic fibroblast growth factor, vascular endothelial growth factor and insulin-like growth factor-1 is beneficial to the migration of BMSCs, which can provide reference of the research of micro environmental conditions of regenerative medicine.