CAJ | 학술논문

预处理是利用木质纤维素转化生成乙醇的关键步骤。实验基于Box-Behnken试验设计，应用Design Expert 8．0软件对玉米芯预处理条件进行响应面分析，建立以同步糖化发酵终点乙醇浓度为响应值的数学模型，结果表明：最佳预处理条件温度为88．6℃、时间为19．81 h、液固比为6．5、硫酸浓度为1．86％（ v/v）。与未预处理的玉米芯相比，经预处理后的固体物料中半纤维素去除率达到77．58％，纤维素含量提高66．49％，利用自絮凝酵母SPSC01进行同步糖化发酵，终点乙醇浓度为12．4 g/L，接近预测值12．64 g/L。当加酶量为35 FPU/g时，发酵终点乙醇浓度可达20．54 g/L。
예처리시이용목질섬유소전화생성을순적관건보취。실험기우Box-Behnken시험설계，응용Design Expert 8．0연건대옥미심예처리조건진행향응면분석，건립이동보당화발효종점을순농도위향응치적수학모형，결과표명：최가예처리조건온도위88．6℃、시간위19．81 h、액고비위6．5、류산농도위1．86％（ v/v）。여미예처리적옥미심상비，경예처리후적고체물료중반섬유소거제솔체도77．58％，섬유소함량제고66．49％，이용자서응효모SPSC01진행동보당화발효，종점을순농도위12．4 g/L，접근예측치12．64 g/L。당가매량위35 FPU/g시，발효종점을순농도가체20．54 g/L。
Pretreatment of corncobs is critical for cellulosic ethanol production. In this study, response surface methodology was used to optimize the pretreatment conditions of corncobs, mathematical model of regarding the ethanol concentration as response value was established. The optimizing experiments were established on the basis of Box-Behnken Design. The results indicated that the optimum conditions were 88.6℃ temperature, 19.8 h, of 6.5∶1 liquid-to-solid ratio, and 1.86%( v/v) H2 SO4 concentration. After such pretreatment, corncobs cellulose content was 53.36%, which was 66.49% higher than the untreated ones, and the hemicellulose removal rate reached to 77?58%. The ethanol concentration was 12.4 g/L after simultaneous saccharification and fermentation by SPSC01, which was very close to the predicted value 12.64 g/L. The ethanol concentration could increase to 20.54 g/L, when the added enzyme dosage was 35 FPU/g.