CAJ | 학술논문

泛素化途径在植物应答生物和非生物胁迫中起着重要作用，其中E3连接酶在泛素化途径中起着决定性作用。室通过在线基因芯片预测发现2个与非生物胁迫相关的拟南芥RING结构域蛋白基因At2g24480（ HHR1）、At5g43200（ HHR2）通过体外泛素化实验证明了二者具有E3连接酶活性。对这两个E3连接酶进行了亚细胞定位，表达谱和组织特异性分析，发现二者受诱导后的表达情况、组织特异性、亚细胞定位均不同。HHR1定位于细胞膜上，HHR2定位于细胞核；表达谱分析表明HHR1响应热和H2 O2胁迫，而HHR2响应盐和H2 O2胁迫；HHR1在根和花中的表达量较高，在茎和叶中的表达量较低，而HHR2在根、茎、叶中的表达量都较高，在花中表达量较低。该研究结果证实了HHR1和HHR2的一些性质和亚细胞定位，为深入研究其功能奠定了基础。
범소화도경재식물응답생물화비생물협박중기착중요작용，기중E3련접매재범소화도경중기착결정성작용。실통과재선기인심편예측발현2개여비생물협박상관적의남개RING결구역단백기인At2g24480（ HHR1）、At5g43200（ HHR2）통과체외범소화실험증명료이자구유E3련접매활성。대저량개E3련접매진행료아세포정위，표체보화조직특이성분석，발현이자수유도후적표체정황、조직특이성、아세포정위균불동。HHR1정위우세포막상，HHR2정위우세포핵；표체보분석표명HHR1향응열화H2 O2협박，이HHR2향응염화H2 O2협박；HHR1재근화화중적표체량교고，재경화협중적표체량교저，이HHR2재근、경、협중적표체량도교고，재화중표체량교저。해연구결과증실료HHR1화HHR2적일사성질화아세포정위，위심입연구기공능전정료기출。
Ubiquitination pathway plays important roles in plant responses to biotic and abiotic stress, especially E3 plays decisive role in pathway of ubiquitin ligases. 2 Arabidopsis RING domain protein At2g24480 ( HHR1 ) and At5g43200( HHR2) were found, which were related to abiotic stress, through prediction of gene chip online. In vitro self-ubiquitination assay demonstrated that the 2 RING domain proteins possess an E3 ligase activity. In this paper, sub-cellular localization, gene expression profiles, tissue specificity of them were conducted. We found that they showed differences in sub-cellular localization, gene expression profiles, tissue specificity. HHR1 was positioned on cell membrane, while HHR2 in nucleus. Our studies demonstrated that both HHR1 and HHR2 were related to H2 O2 stress. In addition, HHR1 and HHR2 were related to heat and salt stress, respectively. The analysis of expressional pattern revealed that HHR1 was highly expressed in root and flower, less expressed in leaves and stems. However, HHR2 relatively expressed less in flower, but highly expressed in roots, stems and leaves. These results confirmed that certain properties and sub-cellular localization of HHR1 and HHR2, and laid foundation for further studies on their function.