CAJ | 학술논문

枯草芽胞杆菌是一种生物安全的革兰氏阳性细菌，在营养匮乏的环境下，可形成具有强抗逆性的芽胞。枯草芽胞杆菌的芽胞由核心、皮层、孢子外套蛋白三部分组成，目前已成功利用枯草芽胞杆菌芽胞外套蛋白CotB、CotC、CotG、CotX和OxdD为载体，将酶蛋白、抗原蛋白或荧光标记蛋白等展示于芽胞表面。芽胞表面展示的蛋白通常具有较好的稳定性、易于纯化和安全性好等优点，可应用于医药、食品及饲料工业等领域，具有较大的应用前景。详细介绍了枯草芽胞杆菌芽胞的分子特点及芽胞表面展示系统的构建过程及其应用前景，为芽胞表面展示载体的基础及应用研究奠定基础。
고초아포간균시일충생물안전적혁란씨양성세균，재영양궤핍적배경하，가형성구유강항역성적아포。고초아포간균적아포유핵심、피층、포자외투단백삼부분조성，목전이성공이용고초아포간균아포외투단백CotB、CotC、CotG、CotX화OxdD위재체，장매단백、항원단백혹형광표기단백등전시우아포표면。아포표면전시적단백통상구유교호적은정성、역우순화화안전성호등우점，가응용우의약、식품급사료공업등영역，구유교대적응용전경。상세개소료고초아포간균아포적분자특점급아포표면전시계통적구건과정급기응용전경，위아포표면전시재체적기출급응용연구전정기출。
Bacillus subtilis is Gram-positive bacteria with biological safy. It can form spores with strong stress resistance in poor nutrient environment. The spore of Bacillus subtilis consists of 3 parts including the core, cortex and spore coat protein. Recently, the Bacillus subtilis spore coat proteins, such as CotB, CotC, CotG, CotX and OxdD, have been successfully used as vectors to display the antigen proteins, enzymes or reporter protein on the spore surfaces. The recombinant proteins on Bacillus subtilis spores usually have many advantages, such as good stability, easy purification and safety. Therefore, they can be used in medicine, food and feed industry, and other fields. It has a great application prospect. This review introduced in detail the molecular characteristics of Bacillus subtilis spores, the construction process of the expressive system on spore surface and its application prospect. Thus, the paper provided a foundation for the basic and applied research about spore surface display system.