听力学及言语疾病杂志
聽力學及言語疾病雜誌
은역학급언어질병잡지
JOURNAL OF AUDIOLOGY AND SPEECH PATHOLOGY
2013年
5期
515-518
,共4页
黄河银%刘砚星%徐鸥%王玉杏%路虹
黃河銀%劉硯星%徐鷗%王玉杏%路虹
황하은%류연성%서구%왕옥행%로홍
听觉剥夺%听皮层%形态学%蛋白酪氨酸磷酸酶2型
聽覺剝奪%聽皮層%形態學%蛋白酪氨痠燐痠酶2型
은각박탈%은피층%형태학%단백락안산린산매2형
Auditory deprivation%Auditory cortex%Morphological change%SHP-2
目的研究双侧耳蜗毁损后大鼠听皮层神经元细胞的形态学变化和蛋白酪氨酸磷酸酶2型(src-homology domain containing protein tygosine phosphatase type 2,SHP -2)基因的表达。方法选取SD大鼠48只,随机分为4个实验组(2周组、4周组、6周组、8周组)和4个相应的对照组,每组6只。实验组动物行双侧耳蜗损毁术,通过HE染色和Nissl染色观察听皮层神经元细胞的形态学变化,用RT -PCR技术检测各组听皮层神经元细胞的SHP-2基因的表达,行相对定量分析。结果 HE染色和Nissl染色可见各实验组大鼠听皮层神经元细胞的凋亡形态随时间延长而加重,呈多样化表现;各对照组大鼠听皮层细胞形态正常。实验2、4、6、8周组SHP-2基因的RT -PCR相对表达量分别为1.1±0.28、1.5±0.04、2.5±0.08、11.0±0.06,随时间延长呈上升趋势,各实验组之间差异均有统计学意义(P<0.05)。结论听觉剥夺可导致听皮层神经元细胞凋亡,凋亡的程度随时间延长逐渐加重;SHP-2基因可促进听皮层神经元细胞的增生,增生的程度也随时间延长而加重;在这两个相互拮抗的因素中,凋亡是最终的结果。
目的研究雙側耳蝸燬損後大鼠聽皮層神經元細胞的形態學變化和蛋白酪氨痠燐痠酶2型(src-homology domain containing protein tygosine phosphatase type 2,SHP -2)基因的錶達。方法選取SD大鼠48隻,隨機分為4箇實驗組(2週組、4週組、6週組、8週組)和4箇相應的對照組,每組6隻。實驗組動物行雙側耳蝸損燬術,通過HE染色和Nissl染色觀察聽皮層神經元細胞的形態學變化,用RT -PCR技術檢測各組聽皮層神經元細胞的SHP-2基因的錶達,行相對定量分析。結果 HE染色和Nissl染色可見各實驗組大鼠聽皮層神經元細胞的凋亡形態隨時間延長而加重,呈多樣化錶現;各對照組大鼠聽皮層細胞形態正常。實驗2、4、6、8週組SHP-2基因的RT -PCR相對錶達量分彆為1.1±0.28、1.5±0.04、2.5±0.08、11.0±0.06,隨時間延長呈上升趨勢,各實驗組之間差異均有統計學意義(P<0.05)。結論聽覺剝奪可導緻聽皮層神經元細胞凋亡,凋亡的程度隨時間延長逐漸加重;SHP-2基因可促進聽皮層神經元細胞的增生,增生的程度也隨時間延長而加重;在這兩箇相互拮抗的因素中,凋亡是最終的結果。
목적연구쌍측이와훼손후대서은피층신경원세포적형태학변화화단백락안산린산매2형(src-homology domain containing protein tygosine phosphatase type 2,SHP -2)기인적표체。방법선취SD대서48지,수궤분위4개실험조(2주조、4주조、6주조、8주조)화4개상응적대조조,매조6지。실험조동물행쌍측이와손훼술,통과HE염색화Nissl염색관찰은피층신경원세포적형태학변화,용RT -PCR기술검측각조은피층신경원세포적SHP-2기인적표체,행상대정량분석。결과 HE염색화Nissl염색가견각실험조대서은피층신경원세포적조망형태수시간연장이가중,정다양화표현;각대조조대서은피층세포형태정상。실험2、4、6、8주조SHP-2기인적RT -PCR상대표체량분별위1.1±0.28、1.5±0.04、2.5±0.08、11.0±0.06,수시간연장정상승추세,각실험조지간차이균유통계학의의(P<0.05)。결론은각박탈가도치은피층신경원세포조망,조망적정도수시간연장축점가중;SHP-2기인가촉진은피층신경원세포적증생,증생적정도야수시간연장이가중;재저량개상호길항적인소중,조망시최종적결과。
Objective To investigate the morphological change and SHP -2(src -homology domain contai-ning protein tygosine phosphatase type 2) gene expression in auditory cortical neuron after auditory deprivation in rat .Methods A total of 48 SD rats were randomly divided into 2-week group ,4-week group ,6-week group ,8-week group and 4 corresponding control groups with 6 rats in each group .Bilateral cochlear ablation was done to traumatized groups to ensure their postoperative ABR threshold was above 90 dB SPL .Then paraffin sectioning and HE and Nissl staining were used to detect morphological change of auditory cortical neuron ,simultaneously the RT-PCR was used to measure the expression of SHP -2 gene in auditory cortical neuron of each group ,and relative quantitative analysis was used .Results The HE and Nissl staining revealed that apoptotic shape of auditory cortical neuron became serious by time ,with the diversified cellular morphology .The relative quantity of SHP -2 gene showed statistical differences between any two groups by the method of one -way ANOVA ,showing a rising trend by time (P<0 .05) .Conclusion Along with time ,auditory cortex ,auditory deprivation showed increasing neuronic apoptosis and neuronic proliferation .Apoptosis was the final result from the mutual antagonism by the 2 factors .