听力学及言语疾病杂志
聽力學及言語疾病雜誌
은역학급언어질병잡지
JOURNAL OF AUDIOLOGY AND SPEECH PATHOLOGY
2013年
5期
509-514
,共6页
韩浩伦%吴玮%余萌%王方园%屈昌北%张建中%王鸿南%李保卫%王刚%孟令照%虞学军%吴大蔚%牛聪敏%刘钢
韓浩倫%吳瑋%餘萌%王方園%屈昌北%張建中%王鴻南%李保衛%王剛%孟令照%虞學軍%吳大蔚%牛聰敏%劉鋼
한호륜%오위%여맹%왕방완%굴창북%장건중%왕홍남%리보위%왕강%맹령조%우학군%오대위%우총민%류강
失重%噪声%凋亡%Caspase-3%雌激素
失重%譟聲%凋亡%Caspase-3%雌激素
실중%조성%조망%Caspase-3%자격소
Weightlessness%Noise%Apoptosis%Caspase-3%Estrogen
目的探讨失重及飞船舱内噪声复合因素对豚鼠听功能及内耳凋亡标志物Caspase-3表达的影响，并观察雌激素对上述复合因素暴露后豚鼠听功能损伤及内耳细胞凋亡的防治作用。方法36只豚鼠随机分为4组，正常对照组6只（对照组），失重＋噪声组（A组）、雌激素治疗组（B组）、雌激素预防组（C组）各10只。各实验组动物均后肢悬吊模拟失重，同时模拟飞船舱内噪声暴露5天，B组暴露过程中每天肌注苯甲酸雌二醇0．08 m g/kg ，首剂加倍，至暴露结束后3天；C组暴露前3天开始肌注苯甲酸雌二醇0．08 mg/kg ，首剂加倍，持续3天。对照组不给任何处理。于实验前、暴露后即刻和恢复3天时行听性脑干反应测试，免疫组织化学染色方法观察凋亡标志物Caspase-3在内耳毛细胞、血管纹及螺旋神经节细胞上的表达。结果暴露后当天，B组ABR反应阈低于A组和C组（P＜0．05）；暴露前后 B组ABR阈移也低于A组和C组（P＜0．05）；恢复前后，C组ABR阈移高于A组低于B组（P＜0．05）。对照组毛细胞、血管纹和螺旋神经节Caspase-3表达均为阴性；A、B、C组在暴露后即刻、恢复3天时，毛细胞、血管纹和螺旋神经节上Caspase-3的表达均为阳性；暴露后即刻，B组毛细胞和血管纹Caspase-3表达较弱，而C组表达较强；恢复3天后，A、B、C三组毛细胞、血管纹和螺旋神经节Caspase-3表达仍为阳性， C组表达较弱。结论使用雌激素治疗可有效减少失重和噪声复合作用暴露后即刻的听力损失和内耳细胞凋亡；而预防性使用雌激素对暴露后即刻的听力损伤无保护作用，但在恢复过程中可促进其恢复，减少内耳细胞凋亡。
目的探討失重及飛船艙內譟聲複閤因素對豚鼠聽功能及內耳凋亡標誌物Caspase-3錶達的影響，併觀察雌激素對上述複閤因素暴露後豚鼠聽功能損傷及內耳細胞凋亡的防治作用。方法36隻豚鼠隨機分為4組，正常對照組6隻（對照組），失重＋譟聲組（A組）、雌激素治療組（B組）、雌激素預防組（C組）各10隻。各實驗組動物均後肢懸弔模擬失重，同時模擬飛船艙內譟聲暴露5天，B組暴露過程中每天肌註苯甲痠雌二醇0．08 m g/kg ，首劑加倍，至暴露結束後3天；C組暴露前3天開始肌註苯甲痠雌二醇0．08 mg/kg ，首劑加倍，持續3天。對照組不給任何處理。于實驗前、暴露後即刻和恢複3天時行聽性腦榦反應測試，免疫組織化學染色方法觀察凋亡標誌物Caspase-3在內耳毛細胞、血管紋及螺鏇神經節細胞上的錶達。結果暴露後噹天，B組ABR反應閾低于A組和C組（P＜0．05）；暴露前後 B組ABR閾移也低于A組和C組（P＜0．05）；恢複前後，C組ABR閾移高于A組低于B組（P＜0．05）。對照組毛細胞、血管紋和螺鏇神經節Caspase-3錶達均為陰性；A、B、C組在暴露後即刻、恢複3天時，毛細胞、血管紋和螺鏇神經節上Caspase-3的錶達均為暘性；暴露後即刻，B組毛細胞和血管紋Caspase-3錶達較弱，而C組錶達較彊；恢複3天後，A、B、C三組毛細胞、血管紋和螺鏇神經節Caspase-3錶達仍為暘性， C組錶達較弱。結論使用雌激素治療可有效減少失重和譟聲複閤作用暴露後即刻的聽力損失和內耳細胞凋亡；而預防性使用雌激素對暴露後即刻的聽力損傷無保護作用，但在恢複過程中可促進其恢複，減少內耳細胞凋亡。
목적탐토실중급비선창내조성복합인소대돈서은공능급내이조망표지물Caspase-3표체적영향，병관찰자격소대상술복합인소폭로후돈서은공능손상급내이세포조망적방치작용。방법36지돈서수궤분위4조，정상대조조6지（대조조），실중＋조성조（A조）、자격소치료조（B조）、자격소예방조（C조）각10지。각실험조동물균후지현조모의실중，동시모의비선창내조성폭로5천，B조폭로과정중매천기주분갑산자이순0．08 m g/kg ，수제가배，지폭로결속후3천；C조폭로전3천개시기주분갑산자이순0．08 mg/kg ，수제가배，지속3천。대조조불급임하처리。우실험전、폭로후즉각화회복3천시행은성뇌간반응측시，면역조직화학염색방법관찰조망표지물Caspase-3재내이모세포、혈관문급라선신경절세포상적표체。결과폭로후당천，B조ABR반응역저우A조화C조（P＜0．05）；폭로전후 B조ABR역이야저우A조화C조（P＜0．05）；회복전후，C조ABR역이고우A조저우B조（P＜0．05）。대조조모세포、혈관문화라선신경절Caspase-3표체균위음성；A、B、C조재폭로후즉각、회복3천시，모세포、혈관문화라선신경절상Caspase-3적표체균위양성；폭로후즉각，B조모세포화혈관문Caspase-3표체교약，이C조표체교강；회복3천후，A、B、C삼조모세포、혈관문화라선신경절Caspase-3표체잉위양성， C조표체교약。결론사용자격소치료가유효감소실중화조성복합작용폭로후즉각적은력손실화내이세포조망；이예방성사용자격소대폭로후즉각적은력손상무보호작용，단재회복과정중가촉진기회복，감소내이세포조망。
Objective To study the effects of compound factors of simulated weightlessness and noise in spaceship on auditory function ,and the expression of apoptotic marker Caspase -3 in inner ear in guinea pigs ,and study the influence of compound factor above on its hearing loss and apoptosis of inner ear .Methods Thirty -six guinea pigs were randomly divided into four groups ,6 in control group ,10 in simulated weightlessness with noise group (Group A) ,treatment estrogen group (Group B) and prevention estrogen (Group C) ,respectively .All the experiment groups were suspended posterior limb ,with the exposure of the simulated noise circumstance in space-ship .Estrogen prevention group received intramuscular injection of estradiol benzoate for 3 days before the experiment ,while estrogen treatment group was treated in the same way for 8 days from the beginning of experiment to 3 days after the experiment .Bilateral auditory brainstem response thresholds (ABR) were tested in pre- exposure ,post-exposure and 3 -day recovery respectively .Cochleae were obtoined for immunohistochemistry staining to observe the expression of the apoptotic marker Caspase -3 in inner ear .Results At the time point of post -exposure ,ABR threshold in Group B was lower than those in Group A by 14 .5 dB and Group C by 9 dB .For the ABR change of pre ,and post-exposure ,Group B was lower 12 .75 dB than those of in Group A and 7 .75 dB than in Group C .For the ABR change between pre ,and post-recovery ,ABR threshold of Group C was higher than those of in Group A by 14 .5 dB and in Group B by 7 dB .These differences were significant (P<0 .05) .The expression of Caspase-3 was positive in the hair cell ,spiral ganglion cells and stria vascularis of three groups in both post exposure and 3-day recovery .The expression of Caspase-3 in hair cell and stria vascularis was strongly positive in spiral ganglion cells in Group C in post -exposure ,while it was slightly positive in Group B .After 3 -day recovery ,Group A turned to negative ,while Group B and Group C were still in positive expression with an increase of that in post -ex-posure .Conclusion Estrogen treatment could effectively reduce the hearing loss and the apoptosis of inner ear in-duced by the compound factor of weightlessness and noise .Preventive use of estrogen could not produce an instant protection on the hearing loss induced by exposure ,and the apoptosis was enhanced .However ,it speeded up the procedure of recovery and reduced the apoptosis of inner ear .
