CAJ | 학술논문

目的：探讨尖吻蝮蛇毒血浆蛋白C激活物（ protein C activator，PCA）在急性心肌梗死诊断中的应用。方法：30只雄性SD大鼠随机分为3组（n＝10），即对照组（C group）、假手术组（S group）和急性心肌梗死组（AMI group）；通过大鼠左冠状动脉前降支结扎术制作急性心肌梗死模型，用Medlab生物信号采集系统检测心电变化。发色底物法测定血浆蛋白C的活性，酶联免疫吸附法（ ELISA）检测血管内皮细胞蛋白C受体（EPCR）的含量，凝血仪检测活化部分凝血活酶时间（ APTT）。结果：AMI组心电显示ST段弓背向上抬高，QRS波群形态改变，T波增高；与S组相比较，AMI组大鼠血浆蛋白C活性1 h即显著降低（ P＜0．05）；2 h后EPCR含量明显减少（ P ＜0．05）；APTT 明显缩短（ P ＜0．05）。结论：以尖吻蝮蛇毒PCA制备的血浆蛋白C活性检测试剂可较灵敏地反映心肌梗死大鼠血浆蛋白C活性的变化。
목적：탐토첨문복사독혈장단백C격활물（ protein C activator，PCA）재급성심기경사진단중적응용。방법：30지웅성SD대서수궤분위3조（n＝10），즉대조조（C group）、가수술조（S group）화급성심기경사조（AMI group）；통과대서좌관상동맥전강지결찰술제작급성심기경사모형，용Medlab생물신호채집계통검측심전변화。발색저물법측정혈장단백C적활성，매련면역흡부법（ ELISA）검측혈관내피세포단백C수체（EPCR）적함량，응혈의검측활화부분응혈활매시간（ APTT）。결과：AMI조심전현시ST단궁배향상태고，QRS파군형태개변，T파증고；여S조상비교，AMI조대서혈장단백C활성1 h즉현저강저（ P＜0．05）；2 h후EPCR함량명현감소（ P ＜0．05）；APTT 명현축단（ P ＜0．05）。결론：이첨문복사독PCA제비적혈장단백C활성검측시제가교령민지반영심기경사대서혈장단백C활성적변화。
Objective:To assess the protein C activator ( PCA) extracted from the venom of Agkistrodon acutus in diagnosis of acute myocardial in-farction( AMI).Methods:Thirty SD male rats were equally randomized into groups of controls ( C group ) , sham operation ( S group ) and AMI ( AMI group) .Animal models of AMI were developed through ligation of the left anterior descending of the coronary artery , and examined for the changes of electrocardiogram with Medlab bio-signal operating system. Chromogenic substrate assay was performed to detect the PC activities ,and enzyme-linked immunosorbent assay( ELISA) was carried out to measure the plasma content of endothelial cell protein C receptor(EPCR).Auto-matic coagulation analyzer was used to determine the activated partial thromboplastin time ( APTT ) .Results:The echocardiographic recording revealed that ST segment and T wave were significant elevated after induc-tion of AMI.Significantly decreased PCA activity at 1 h,reduced EPCR level at 2 h and shortened APTT were observed in AMI group as compared with S group(P<0.05).Conclusion:PCA kit prepared from the venom of Agkistrodon acutus may sensitively indicate the changes of serum PC in rats with AMI.