内蒙古民族大学学报（自然科学版）
內矇古民族大學學報（自然科學版）
내몽고민족대학학보（자연과학판）
JOURNAL OF INNER MONGOLIA UNIVERSITY FOR NATIONALITIES(NATURAL SCIENCES)
2013年
5期
568-571
,共4页
散丹%贾海侠%嘎比日%白玉霞
散丹%賈海俠%嘎比日%白玉霞
산단%가해협%알비일%백옥하
蒙药胡日查-6味丸%没食子酸%HPLC法%含量测定
矇藥鬍日查-6味汍%沒食子痠%HPLC法%含量測定
몽약호일사-6미환%몰식자산%HPLC법%함량측정
Huricha-6 flavored pills%Gallic acid%HPLC%Content determination
目的：建立蒙药胡日查-6味丸中没食子酸的高效液相色谱含量测定方法.方法：采用反相高效液相色谱法，色谱柱为Diamonsil C18柱（150mm×4.6mm，5μm）；流动相为0.1%磷酸水溶液-甲醇（96：4）；检测波长为273 nm；流速为1.0ml/min，柱温30℃的条件下进行含量测定.结果：没食子酸在0.135～0.810μg范围内与峰面积积分值呈良好的线性关系.回归方程为Y=2557.2X-14459，r=0.9997，加样回收率为99.05%，RSD为0.83%（n=6）.结论：本法简便、快捷、重复性好，结果准确可靠，可为研究蒙药胡日查-6味丸的质量标准方法的建立提供可靠依据.
目的：建立矇藥鬍日查-6味汍中沒食子痠的高效液相色譜含量測定方法.方法：採用反相高效液相色譜法，色譜柱為Diamonsil C18柱（150mm×4.6mm，5μm）；流動相為0.1%燐痠水溶液-甲醇（96：4）；檢測波長為273 nm；流速為1.0ml/min，柱溫30℃的條件下進行含量測定.結果：沒食子痠在0.135～0.810μg範圍內與峰麵積積分值呈良好的線性關繫.迴歸方程為Y=2557.2X-14459，r=0.9997，加樣迴收率為99.05%，RSD為0.83%（n=6）.結論：本法簡便、快捷、重複性好，結果準確可靠，可為研究矇藥鬍日查-6味汍的質量標準方法的建立提供可靠依據.
목적：건립몽약호일사-6미환중몰식자산적고효액상색보함량측정방법.방법：채용반상고효액상색보법，색보주위Diamonsil C18주（150mm×4.6mm，5μm）；류동상위0.1%린산수용액-갑순（96：4）；검측파장위273 nm；류속위1.0ml/min，주온30℃적조건하진행함량측정.결과：몰식자산재0.135～0.810μg범위내여봉면적적분치정량호적선성관계.회귀방정위Y=2557.2X-14459，r=0.9997，가양회수솔위99.05%，RSD위0.83%（n=6）.결론：본법간편、쾌첩、중복성호，결과준학가고，가위연구몽약호일사-6미환적질량표준방법적건립제공가고의거.
Objective: To establish the high performance liquid chromatography (HPLC) method for determination of the content of gallic acid in Mongolian medicine Huricha-6 flavored pills. Methods:Diamonsil C18 column (150mm× 4.6mm, 5μm) was adopted, the mobile phase was methanol-0.1%phosphoric acid solution (4:96), detective wave-length was 273 nm, flow rate was 1.0 ml/min, column temperature was 30℃. Results:The correlation curves of gallic acid was linear in the range of 0.135-0.810μg, and the regression equation was Y=2557.2X-14459 (r=0.9997), The average recovery was 99.05%, RSD was 0.83%(n=6). Conclusion:The method is easy, rapid and reproducible for quality control of Huricha-6 pills.
