内蒙古民族大学学报（自然科学版）
內矇古民族大學學報（自然科學版）
내몽고민족대학학보（자연과학판）
JOURNAL OF INNER MONGOLIA UNIVERSITY FOR NATIONALITIES(NATURAL SCIENCES)
2013年
5期
532-535,564
,共5页
蓖麻%连锁遗传图%SSR
蓖痳%連鎖遺傳圖%SSR
비마%련쇄유전도%SSR
Castor%Linkage map%SSR
以YC1×YF181自交形成的161个F2单株为作图群体，利用345对SSR引物和30对ISSR选扩引物,在亲本YC1和YF181之间进行了多态性检测，筛选出80对SSR和1对ISSR引物用于F2群体分析.利用上述引物组合共检测到145个多态性标记位点，其中的124个标记构建了蓖麻分子连锁图谱该图谱覆盖基因组全长396 cM，平均图距7.76cM.
以YC1×YF181自交形成的161箇F2單株為作圖群體，利用345對SSR引物和30對ISSR選擴引物,在親本YC1和YF181之間進行瞭多態性檢測，篩選齣80對SSR和1對ISSR引物用于F2群體分析.利用上述引物組閤共檢測到145箇多態性標記位點，其中的124箇標記構建瞭蓖痳分子連鎖圖譜該圖譜覆蓋基因組全長396 cM，平均圖距7.76cM.
이YC1×YF181자교형성적161개F2단주위작도군체，이용345대SSR인물화30대ISSR선확인물,재친본YC1화YF181지간진행료다태성검측，사선출80대SSR화1대ISSR인물용우F2군체분석.이용상술인물조합공검측도145개다태성표기위점，기중적124개표기구건료비마분자련쇄도보해도보복개기인조전장396 cM，평균도거7.76cM.
161 F2 individuals derived from the single cross of inbred lines YC1 and YF181 were used as mapping popu-lation in castor. 345 SSR primer-pairs were used to detect the polymorphisms between parents YC1 and YF181. 80 SSR and 1 ISSR primer-pairs were selected to analyze F2 population. 145 markers were detected by the primer-pairs mentioned above. Only 124 markers were used to construct the molecular genetic linkage map, which span castor ge-nome about 396cM, with average distance of 7.76 cM between markers.
