CAJ | 학술논문

目的：探讨B细胞易位基因1（ BTG1）在人肾小管上皮细胞株（ HK-2）和肾透明细胞腺癌细胞株（786-O）中的表达及BTG1基因对HK-2和786-O细胞周期、细胞凋亡的影响。方法：采用Western blotting方法检测BTG1蛋白在HK-2、786-O细胞株中的表达，构建BTG1表达质粒转染786-O细胞，合成BTG1干扰RNA转染HK-2细胞，通过流式细胞仪（ FACS）观察BTG1对HK-2、786-O细胞周期和细胞凋亡的影响。结果：Western blotting检测显示，BTG1蛋白在HK-2中的表达高于786-O。786-O中高表达BTG1蛋白能够促进其凋亡，增加其G0/G1期比率；HK-2中低表达BTG1蛋白能够抑制其凋亡，减少其G0/G1期比率。结论：BTG1可能通过对细胞凋亡及细胞周期的调控抑制肾细胞癌的生长。
목적：탐토B세포역위기인1（ BTG1）재인신소관상피세포주（ HK-2）화신투명세포선암세포주（786-O）중적표체급BTG1기인대HK-2화786-O세포주기、세포조망적영향。방법：채용Western blotting방법검측BTG1단백재HK-2、786-O세포주중적표체，구건BTG1표체질립전염786-O세포，합성BTG1간우RNA전염HK-2세포，통과류식세포의（ FACS）관찰BTG1대HK-2、786-O세포주기화세포조망적영향。결과：Western blotting검측현시，BTG1단백재HK-2중적표체고우786-O。786-O중고표체BTG1단백능구촉진기조망，증가기G0/G1기비솔；HK-2중저표체BTG1단백능구억제기조망，감소기G0/G1기비솔。결론：BTG1가능통과대세포조망급세포주기적조공억제신세포암적생장。
Objective:To explore the expression of B-cell translocation gene 1 ( BTG1 ) in normal renal tubule epithelial cell lines (HK-2) and renal clear cell cancer cell lines (786-O) and its influence on cell cycle and apoptosis of HK-2 and 786-O.Methods: The expression of BTG1 in HK-2 and 786-O was detected by Western blotting.The BTG1 expression plasmid was constructed and transfected into 786-O.The interference RNA of BTG1 was designed and transfected into HK-2.The effect of BTG1 on cell cycle and apoptosis of HK-2 and 786-O was observed by flow cytometry (FACS).Results:The expression of BTG1 protein was higher in HK-2 than in 786-O. High expression of BTG 1 would promote cell apoptosis and increase the rate of G 0/G1 phase in 786-O.Low expression of BTG1 would inhibit the cell apoptosis and reduce rate of G 0/G1 phase in HK-2.Conclusion:BTG1 can inhibit the growth of renal cell carcinoma through regulation of cell cycle and apoptosis .