CAJ | 학술논문

目的：探讨大肠癌中错配修复基因（MMR）表达缺失与化疗药物5-氟尿嘧啶（5-FU）药物敏感性的关系。方法选取4株大肠癌细胞株SW480、HT29、Lovo、HCT116进行细胞培养，运用MTT法分析不同细胞株对5-FU药物敏感性，采用流式细胞技术检测细胞周期及凋亡情况。结果 MMR基因缺失的细胞株 Lovo、HCT116对5-FU的敏感性要高于 MMR 基因完整的细胞株 HT29、SW480，并且以 MSI -H 的大肠癌细胞株HCT116的敏感性最高，MSS的大肠癌细胞株HT29敏感性最低。流式细胞术分析Lovo、HT29的细胞周期，5-FU处理细胞后，细胞周期中S期的比例明显减少（ P＜0.05），5-FU作用细胞后抑制了细胞的增殖速度，阻断细胞的DNA合成和复制。结论 MMR基因缺失大肠癌细胞株对5-FU的敏感性明显高于MMR基因完整大肠癌细胞株，不同浓度的5-FU药物与癌细胞之间存在着剂量效应及时间效应关系。
목적：탐토대장암중착배수복기인（MMR）표체결실여화료약물5-불뇨밀정（5-FU）약물민감성적관계。방법선취4주대장암세포주SW480、HT29、Lovo、HCT116진행세포배양，운용MTT법분석불동세포주대5-FU약물민감성，채용류식세포기술검측세포주기급조망정황。결과 MMR기인결실적세포주 Lovo、HCT116대5-FU적민감성요고우 MMR 기인완정적세포주 HT29、SW480，병차이 MSI -H 적대장암세포주HCT116적민감성최고，MSS적대장암세포주HT29민감성최저。류식세포술분석Lovo、HT29적세포주기，5-FU처리세포후，세포주기중S기적비례명현감소（ P＜0.05），5-FU작용세포후억제료세포적증식속도，조단세포적DNA합성화복제。결론 MMR기인결실대장암세포주대5-FU적민감성명현고우MMR기인완정대장암세포주，불동농도적5-FU약물여암세포지간존재착제량효응급시간효응관계。
Objective To analyze the correlation between mismatch repair gene ( MMR) expression and chemo-therapeutic drugs 5-fluorouracil (5-FU) sensitivity in colorectal cancer.Methods Four colorectal cancer cell lines SW480, HT29, Lovo and HCT116 were selected and cultured .The 5-FU sensitivity was assessed by MTT , while the cell cycle and apoptosis were observed using flow cytometry (FCM).Results The 5-FU sensitivity was higher in MMR gene silenced cell lines Lovo and HCT 116 than that in complete MMR gene cell lines HT 29, SW480, and MSI -H;among which the highest and lowest sensitivities were observed in the colorectal cancer cell line HCT 116 and MSS colon cancer cell line HT29, respectively.According to cell cycle analysis , significant reduction in S phase cell percentage was observed in 5-FU treated Lovo and HT29 cells ( P<0.05 ) .Conclusion MMR gene silencing significantly increases the 5-FU sensitivity in human colorectal cancer cell lines .Furthermore, 5-FU inhibits the cancer cell proliferation in dose-and time-dependent manners .