CAJ | 학술논문

目的：根据ATP核酸适配子与ATP结合的能力强于其互补核酸的原理，构建基于核酸适配子的高灵敏的ATP检测方法。方法：以ATP核酸适配子作为识别分子，对检测的捕获探针固定浓度、杂交时间、ATP反应时间、银染增强时间等进行优化，在优化的反应条件下，对其敏感性、特异性进行评价。结果：在优化的反应条件下，通过对不同浓度的ATP样本进行检测，发现ATP浓度在10-14M至10-9M 范围内时， ATP浓度的对数与灰度值的相对强度呈线性关系，相关系数为0．996。并且，在优化的反应条件下，该方法对ATP的检测具有较高的特异性。结论：所构建的基于核酸适配子的ATP检测方法具有灵敏度高、特异性高的优点，可为其他生物分子的检测提供新的思路。
목적：근거ATP핵산괄배자여ATP결합적능력강우기호보핵산적원리，구건기우핵산괄배자적고령민적ATP검측방법。방법：이ATP핵산괄배자작위식별분자，대검측적포획탐침고정농도、잡교시간、ATP반응시간、은염증강시간등진행우화，재우화적반응조건하，대기민감성、특이성진행평개。결과：재우화적반응조건하，통과대불동농도적ATP양본진행검측，발현ATP농도재10-14M지10-9M 범위내시， ATP농도적대수여회도치적상대강도정선성관계，상관계수위0．996。병차，재우화적반응조건하，해방법대ATP적검측구유교고적특이성。결론：소구건적기우핵산괄배자적ATP검측방법구유령민도고、특이성고적우점，가위기타생물분자적검측제공신적사로。
Objective :According to the principle that the combining capacity of ATP aptamer with ATP is stronger than its complementary nucleic acid ,we built high sensitive detection method for ATP based on aptamer .Methods :ATP aptamer was taken as recognition molecule ,we optimized reaction conditions of the capture probe fixed concentration , hybridization time ,ATP response time and silver enhancement time , and evaluated the sensitivity and specificity of this method .Results :Tested the different concentrations of ATP under the optimal conditions ,we found that the relative intensity of the greyscale was proportional to the logarithmic value of ATP concentration over a 5-decade range from 10-14M to 10 -9M with a linear correlation coefficient of 0 .996 .Under the optimal conditions ,the proposed method has high specificity for ATP detection .Conclusion :This study built a aptamer based ATP detection method with high specificity and high sensitivity ,and can provide a fresh perspective for the detection of other materials .