CAJ | 학술논문

目的：观察局灶脑缺血/再灌注后不同阶段大鼠海马内IKKα及NF-κBp52的表达及电针对NF -κB旁路途径的调节作用。方法：SD健康雄性大鼠（280-300g）108只随机分为假手术组（n＝12），电针组（n＝48）及模型组（n＝48），按照再灌注后24h与7d将电针组及模型组分为2个亚组。采用右侧大脑中动脉闭塞/再通线栓法建立局灶脑缺血/再灌注模型。电针组取“合谷”穴、“太冲”穴，选用G6805-1型针灸治疗仪进行电针治疗。用FJB染色观察脑缺血/再灌注后海马区内神经细胞的损伤情况；利用免疫组化、Western blot 检测不同时间点海马区内IKKα的蛋白表达及NF-κB p52胞核内的蛋白表达变化。结果：FJB染色显示局灶脑缺血/再灌注后，海马区内神经细胞变性损伤明显（P＜0．05）；与模型组比较，电针干预后海马区内神经细胞变性损伤减少（P＜0．05）。免疫组化及Western blot 显示模型组大鼠海马内IKKα表达于再灌注后24h及7d均显著升高（P＜0．05）；胞核内NF-κB p52的蛋白表达随IKKα表达增加而增加（P＜0．05）。电针组与模型组比较，海马内IKKα蛋白表达及胞核内p52的蛋白表达明显减少（P＜0．05）。结论：脑缺血/再灌注刺激能诱发海马区内IKKα表达升高，活化p52入核激活信号通路造成海马区内神经细胞的损害；电针干预后能明显抑制IKKα的表达，阻止其活化p52进而有效缓解缺血/再灌注后海马内神经细胞的损伤。
목적：관찰국조뇌결혈/재관주후불동계단대서해마내IKKα급NF-κBp52적표체급전침대NF -κB방로도경적조절작용。방법：SD건강웅성대서（280-300g）108지수궤분위가수술조（n＝12），전침조（n＝48）급모형조（n＝48），안조재관주후24h여7d장전침조급모형조분위2개아조。채용우측대뇌중동맥폐새/재통선전법건립국조뇌결혈/재관주모형。전침조취“합곡”혈、“태충”혈，선용G6805-1형침구치료의진행전침치료。용FJB염색관찰뇌결혈/재관주후해마구내신경세포적손상정황；이용면역조화、Western blot 검측불동시간점해마구내IKKα적단백표체급NF-κB p52포핵내적단백표체변화。결과：FJB염색현시국조뇌결혈/재관주후，해마구내신경세포변성손상명현（P＜0．05）；여모형조비교，전침간예후해마구내신경세포변성손상감소（P＜0．05）。면역조화급Western blot 현시모형조대서해마내IKKα표체우재관주후24h급7d균현저승고（P＜0．05）；포핵내NF-κB p52적단백표체수IKKα표체증가이증가（P＜0．05）。전침조여모형조비교，해마내IKKα단백표체급포핵내p52적단백표체명현감소（P＜0．05）。결론：뇌결혈/재관주자격능유발해마구내IKKα표체승고，활화p52입핵격활신호통로조성해마구내신경세포적손해；전침간예후능명현억제IKKα적표체，조지기활화p52진이유효완해결혈/재관주후해마내신경세포적손상。
Objective :To observe the effect of electroacupuncture (EA) on the IKKαexpression in the hippocampus at the different time points after ischemia /reperfusion .Methods :One hundred and eight healthy male SD rats were randomly divided into sham group ,I/R group and EA group .The I/R group and EA group were further divided respectively into 2 subgroups according to the different duration of reperfusion as 24h and 7d .The focal cerebral ischemia/reperfusion was established by middle cerebral artery occlusion/reperfusion . The G6805 -1 electroacupuncture treatment equipment was used and picked the “Hegu” and“Taichong” acupoints in this research .The degeneration of neuron cells was tested by FJB stain .The IKKαprotein expression and the NF -κB p52 expression in the nucleus were detected by immunohistochemistry and Western blot analysis in the hippocampus at different time points .Results Compared with the sham group , the degeneration of neuron cells in the hippocampus in I/R group was significantly increased (P<0 .05) ,but after the EA treatment ,the neuron cells damage in hippocampus was significantly decreased than that in I/R group (P<0 .05) .The expression of IKKαprotein in I/R group was remarkably increased at 24h and 7d after reperfusion (P<0 .05) .Meanwhile ,the NF-κB p52 protein expression in the nucleus was also significantly increased (P<0 .05) .With EA treatment ,the expression of IKKαand the expression of NF -κB p52 protein expression in the nucleus were significantly decreased (p<0 .05) .Conclusion The IKKα protein expression was increased by focal cerebral ischemia/reperfusion stimulation ,then activated the NF -κB p52 translocation to activate the signaling pathway and caused the neuron cells damage in the hippocampus after focal cerebral ischemia /reperfusion .The EA treatment significantly inhibited the IKKαexpression ,then stopped the activation of p52 to effectively alleviate the injury in the hippocampus after focal cerebral ischemia /reperfusion .