干旱地区农业研究
榦旱地區農業研究
간한지구농업연구
AGRICULTURAL RESEARCH IN THE ARID AREAS
2013年
5期
238-244
,共7页
周芳%刘恩世%赵平娟%王文泉%彭明
週芳%劉恩世%趙平娟%王文泉%彭明
주방%류은세%조평연%왕문천%팽명
木薯%苗期%干旱胁迫%植物内源激素%含量变化
木藷%苗期%榦旱脅迫%植物內源激素%含量變化
목서%묘기%간한협박%식물내원격소%함량변화
cassava%seedling stage%drought stress%endogenous phytohormone%content change
以SC124、KU50、C4和SC8四个木薯品种为试验材料,运用酶联免疫吸附测定法,研究了盆栽条件下干旱胁迫对苗期木薯根系与叶片中内源激素脱落酸(ABA)、吲哚乙酸(IAA)、赤霉素(GA)和玉米素核苷(ZR)的含量以及四种激素间平衡关系的影响。结果表明:随着干旱程度的加强,四种激素的含量在不同木薯品种内呈现不同的变化趋势。干旱至第10天时,ABA含量在苗期木薯叶片内增加,在根系内含量也增加(KU50除外);IAA在叶片中含量下降,根系内含量因品种不同,变化趋势相差明显,其中SC124和KU50中IAA含量显著下降,而在SC8和C4中含量上升;GA含量在四种品种的根系中均下降,叶片GA含量在C4和KU50中略有下降而在SC124和SC8中上升;ZR含量在四种品种的根系中均下降,叶片中ZR含量在C4中上升,SC8中下降,而在KU50和SC124中无明显变化。IAA/ABA ,ZR/ABA在根系中比值下降,SC124降幅最大,SC8降幅最小,KU50、C4降幅居中,说明IAA/ABA ,ZR/ABA与木薯的抗旱能力具有一定相关性。该研究结果解释了苗期木薯根系和叶片内源激素对干旱胁迫的适应性反应,为筛选不同木薯抗旱品种提供了理论依据。
以SC124、KU50、C4和SC8四箇木藷品種為試驗材料,運用酶聯免疫吸附測定法,研究瞭盆栽條件下榦旱脅迫對苗期木藷根繫與葉片中內源激素脫落痠(ABA)、吲哚乙痠(IAA)、赤黴素(GA)和玉米素覈苷(ZR)的含量以及四種激素間平衡關繫的影響。結果錶明:隨著榦旱程度的加彊,四種激素的含量在不同木藷品種內呈現不同的變化趨勢。榦旱至第10天時,ABA含量在苗期木藷葉片內增加,在根繫內含量也增加(KU50除外);IAA在葉片中含量下降,根繫內含量因品種不同,變化趨勢相差明顯,其中SC124和KU50中IAA含量顯著下降,而在SC8和C4中含量上升;GA含量在四種品種的根繫中均下降,葉片GA含量在C4和KU50中略有下降而在SC124和SC8中上升;ZR含量在四種品種的根繫中均下降,葉片中ZR含量在C4中上升,SC8中下降,而在KU50和SC124中無明顯變化。IAA/ABA ,ZR/ABA在根繫中比值下降,SC124降幅最大,SC8降幅最小,KU50、C4降幅居中,說明IAA/ABA ,ZR/ABA與木藷的抗旱能力具有一定相關性。該研究結果解釋瞭苗期木藷根繫和葉片內源激素對榦旱脅迫的適應性反應,為篩選不同木藷抗旱品種提供瞭理論依據。
이SC124、KU50、C4화SC8사개목서품충위시험재료,운용매련면역흡부측정법,연구료분재조건하간한협박대묘기목서근계여협편중내원격소탈락산(ABA)、신타을산(IAA)、적매소(GA)화옥미소핵감(ZR)적함량이급사충격소간평형관계적영향。결과표명:수착간한정도적가강,사충격소적함량재불동목서품충내정현불동적변화추세。간한지제10천시,ABA함량재묘기목서협편내증가,재근계내함량야증가(KU50제외);IAA재협편중함량하강,근계내함량인품충불동,변화추세상차명현,기중SC124화KU50중IAA함량현저하강,이재SC8화C4중함량상승;GA함량재사충품충적근계중균하강,협편GA함량재C4화KU50중략유하강이재SC124화SC8중상승;ZR함량재사충품충적근계중균하강,협편중ZR함량재C4중상승,SC8중하강,이재KU50화SC124중무명현변화。IAA/ABA ,ZR/ABA재근계중비치하강,SC124강폭최대,SC8강폭최소,KU50、C4강폭거중,설명IAA/ABA ,ZR/ABA여목서적항한능력구유일정상관성。해연구결과해석료묘기목서근계화협편내원격소대간한협박적괄응성반응,위사선불동목서항한품충제공료이론의거。
Using four kinds of cassava varieties (SC124 ,KU50 ,C4 and SC8 ) as test materials ,the enzyme-linked immunosorbent assay was conducted to determine the content changes and the balance of endogenous phytohormones ,in-cluding abscisic acid (ABA ) ,indole-acetic acid (IAA ) ,gibberellin (GA ) and aeatin riboside (ZR) ,in roots and leaves of cassava at seedling stage under drought stress .The results showed that ,with the strengthening of drought stress ,the content of various hormones showed different trends in different cassava varieties .At the tenth day of drought stress ,the content of ABA increased in leaves ,so did in roots (except for KU50 );the content of IAA decreased in leaves of all va-rieties ,while significantly decreased in roots of SC124 and KU50 but increased in roots of SC8 and C4;the content of GA decreased in roots of all varieties ,while increased in leaves of SC124 and SC8 but decreased slightly in leaves of C4 and KU50;the content of ZR decreased in roots of all varieties ,while increased in leaves of C4 ,decreased in leaves of SC8 ,but no significant change in leaves of KU 50 and SC124 .The ratio of IAA/ABA and ZR/ABA decreased in roots , in which ,the reduction rate of SC124 was the largest ,that of SC8 was the smallest ,and that of KU50 and C4 was in the middle .This indicated that the ratio of IAA/ABA and ZR/ABA has a certain relevance to drought resistance of cassava . The research explained the adaptive responses of endogenous hormones in roots and leaves of cassava at seedling stage to drought stress ,and provided a theoretical basis for screening drought-resistant varieties of cassava .