CAJ | 학술논문

目的通过检测998例疑似强直性脊柱炎（Ankylosing spondylitis，AS）患者HLA-B27抗原阳性率和表达强度，了解内蒙古地区AS与HLA-B27抗原的相关性，并初步探讨其临床意义。方法采用流式细胞术检测患者外周血T淋巴细胞膜上的HLA-B27抗原和其平均荧光强度（mean fluorescence intensity，MFI）。结果998例疑似AS患者以21~50岁青壮年为主，占77.35%；男女比例为1.4∶1；998例疑似AS患者的HLA-B27抗原阳性率28.86%；男女患者HLA-B27抗原阳性率分别为33.05%和23.02%（P<0.05）；男女阳性患者的MFI有明显差异（P<0.05）。结论内蒙古地区AS患者与HLA-B27抗原有着很强的相关性。采用流式细胞技术检测临床疑似AS患者T淋巴细胞HLA-B27表达强度有助于诊断和鉴别诊断AS。
목적통과검측998례의사강직성척주염（Ankylosing spondylitis，AS）환자HLA-B27항원양성솔화표체강도，료해내몽고지구AS여HLA-B27항원적상관성，병초보탐토기림상의의。방법채용류식세포술검측환자외주혈T림파세포막상적HLA-B27항원화기평균형광강도（mean fluorescence intensity，MFI）。결과998례의사AS환자이21~50세청장년위주，점77.35%；남녀비례위1.4∶1；998례의사AS환자적HLA-B27항원양성솔28.86%；남녀환자HLA-B27항원양성솔분별위33.05%화23.02%（P<0.05）；남녀양성환자적MFI유명현차이（P<0.05）。결론내몽고지구AS환자여HLA-B27항원유착흔강적상관성。채용류식세포기술검측림상의사AS환자T림파세포HLA-B27표체강도유조우진단화감별진단AS。
Objective To investigate the relationship between suspected ankylosing spondylitis and HLA-B27 antigen in Inner Mongolia by detecting the positive rate and expression intensity of HLA-B27 antigen in 998 suspected AS patients, and evaluate its clinical significance. Methods The positive rate and the mean fluorescence intensity (MFI) of HLA-B27 antigen on the peripheral blood T lymphocytic detected by flow cytometer. Results Among the 998 suspected AS patients, the majority patients (77.35%) were aged from 21 to 50 years old, and the ratio between male and female was 1.4∶1. Among the all patients, the positive rate of antigen HLA-B27 was 28.86%, and the male and female patients' positive rates of HLA-B27 antigen were 33.05% and 23.02%, respectively (P<0.05). The MFI of male and female patients were significantly different (P<0.05). Conclusion The patients with AS in Inner Mongolia are strongly associated with HLA-B27 antigen. Detection of HLA-B27 antigen expression intensity in suspected AS patients with FCM is helpful to diagnosis and differential diagnosis of AS.