CAJ | 학술논문

以八棱海棠cDNA为模板，采用RT-PCR、巢式PCR、3’RACE、5’RACE技术，获得了类天冬氨酸特异性的半胱氨酸蛋白水解酶（Caspase）基因cDNA 1429bp的全长序列。序列分析表明，Caspase基因cDNA序列编码358个氨基酸，N端含有1个23个氨基酸的信号肽。进化树分析显示八棱海棠与山杏在该基因座位上亲缘关系最近，属于一个分支。
이팔릉해당cDNA위모판，채용RT-PCR、소식PCR、3’RACE、5’RACE기술，획득료류천동안산특이성적반광안산단백수해매（Caspase）기인cDNA 1429bp적전장서렬。서렬분석표명，Caspase기인cDNA서렬편마358개안기산，N단함유1개23개안기산적신호태。진화수분석현시팔릉해당여산행재해기인좌위상친연관계최근，속우일개분지。
The caspase-like gene cDNA sequence was cloned with cDNA isolated from malus robusta rehd .A 1429bp full length cDNA sequence of caspase-like gene was obtained by using RT-PCR ,nested PCR ,3’ RACE and 5’ RACE techniques . Further analysis on caspase-like gene cDNA indicates that it encoded a peptide containing 358 amino acids ,existing a signal peptide of 23 amino acids in the N terminal .Phylogenetic tree analysis shows malus robusta rehd , and apricot in the Caspase-like gene locus have the closest relationship ,which belongs to a branch .