CAJ | 학술논문

目的探讨左旋—二脱水伊地醇双甲磺酸酯(DMDAI-L)对HL-60细胞Bax蛋白表达的影响。方法通过免疫细胞化学法和western blot分别测定不同剂量DMDAI-L对HL-60细胞的Bax蛋白的表达情况。结果免疫细胞化学法定性实验结果显示药物干预后HL-60细胞Bax阳性表达高于正常对照组；western blot结果显示不同浓度药物（2.5μg/ml、5μg/ml、10μg/ml）干预HL-60细胞48h后，Bax蛋白相对表达(0.59±0.02、0.90±0.09、1.07±0.03)，正常对照组为（0.32±0.03）；各给药组与正常对照组比较均有统计学意义（p<0.05）。结论 DMDAI-L诱导HL-60细胞凋亡过程中，其作用机制可能与上调Bax蛋白表达有关。
목적탐토좌선—이탈수이지순쌍갑광산지(DMDAI-L)대HL-60세포Bax단백표체적영향。방법통과면역세포화학법화western blot분별측정불동제량DMDAI-L대HL-60세포적Bax단백적표체정황。결과면역세포화학법정성실험결과현시약물간예후HL-60세포Bax양성표체고우정상대조조；western blot결과현시불동농도약물（2.5μg/ml、5μg/ml、10μg/ml）간예HL-60세포48h후，Bax단백상대표체(0.59±0.02、0.90±0.09、1.07±0.03)，정상대조조위（0.32±0.03）；각급약조여정상대조조비교균유통계학의의（p<0.05）。결론 DMDAI-L유도HL-60세포조망과정중，기작용궤제가능여상조Bax단백표체유관。
Objective The aim of this present research was to investigate the expression of the apoptosis-related protein Bax in HL-60 cells after treatment with DMDAI-L.Methods HL-60 was treated with different doses of DMDAI-L for 48 hours. The protein of Bax was determined by immunocytochemical method and western blot analysis.Results The positive expression of Bax was higher than control group after treatment with DMDAI-L about 48 hours;For western blot ,the ratio of Bax protein were (0.59±0.02、 0.90±0.09 、1.07±0.03) and the control one was (0.32±0.03). There was significant difference between control group and experience groups (p<0.05).Conclusion The ability of DMDAI-L induced HL-60 cells apoptosis may be related to increase Bax protein expression level.