CAJ | 학술논문

通过温室水培实验，研究在不同锰质量浓度(2.5、50、100、150、200、300 mg·L-1)胁迫下青葙(Celosia argentea Linn.)的叶绿素含量、叶绿素荧光参数的变化，及对其生长的影响，探究青葙对锰毒的耐性。结果表明，随着Mn2+质量浓度的升高，青葙叶片的叶绿素含量和叶绿素荧光参数值都出现不同程度下降。当 Mn处理浓度大于50 mg·L-1时，叶绿素a、叶绿素 b 含量和总叶绿素含量显著下降，但在 Mn 质量浓度为100-300 mg·L-1的范围内，叶绿素 b 含量变化不明显(p>0.05)。青葙的最大光化学量子产量(Fv/Fm)、最大荧光产量(Fm)、最小荧光产量(Fo)、光合量子产量(EQY)、表观光合电子传递速率(ETR)、光合化学淬灭系数(qP)均随 Mn2+质量浓度的增加显著降低，说明锰胁迫抑制了青葙的电子传递过程。但在150、200、300 mg·L-1质量浓度锰处理下 ETR、EQY、qP下降趋势变缓，且 ETR、EQY不存在显著差异。青葙株高与 ETR、EQY 的变化规律基本相似，根长则与对照组相比下降不显著，到300 mg·L-1才出现明显降低。试验结果证明青葙对锰毒具有耐性。
통과온실수배실험，연구재불동맹질량농도(2.5、50、100、150、200、300 mg·L-1)협박하청상(Celosia argentea Linn.)적협록소함량、협록소형광삼수적변화，급대기생장적영향，탐구청상대맹독적내성。결과표명，수착Mn2+질량농도적승고，청상협편적협록소함량화협록소형광삼수치도출현불동정도하강。당 Mn처리농도대우50 mg·L-1시，협록소a、협록소 b 함량화총협록소함량현저하강，단재 Mn 질량농도위100-300 mg·L-1적범위내，협록소 b 함량변화불명현(p>0.05)。청상적최대광화학양자산량(Fv/Fm)、최대형광산량(Fm)、최소형광산량(Fo)、광합양자산량(EQY)、표관광합전자전체속솔(ETR)、광합화학쉬멸계수(qP)균수 Mn2+질량농도적증가현저강저，설명맹협박억제료청상적전자전체과정。단재150、200、300 mg·L-1질량농도맹처리하 ETR、EQY、qP하강추세변완，차 ETR、EQY불존재현저차이。청상주고여 ETR、EQY 적변화규률기본상사，근장칙여대조조상비하강불현저，도300 mg·L-1재출현명현강저。시험결과증명청상대맹독구유내성。
Celosia argentea Linn., an annual herb, was found to be a pioneer species on the Mn mine wasteland. To gain fundamental insights into the tolerance of C. argentea to Mn excess, 28-days-old plants grown in aerated nutrient solution were supplied with 2.5(control), 50, 100, 150, 200 and 300 mg·L-1 Mn and analyzed for chlorophyll contents, chlorophyll fluorescence parameters, height and root length. It was found that chlorophyll contents and chlorophyll fluorescence parameters were decreased with increased in Mn supply level. Chlorophyll a, chlorophyll b and total chlorophyll contents dropped significantly as Mn concentrations exceeded 50 mg·L-1; however, the contents of chlorophyll b did not significantly decreased at Mn concentrations ranging from 100 mg·L-1 to 300 mg·L-1 (p>0.05). The responses of chlorophyll fluorescence parameters in leaves of C. argentea showed that maximum quantum yield (Fv/Fm), maximum fluorescence(Fm), minimal fluorescence (Fo), effective quantum yield (EQY), electron transport rate (ETR) and photo chemical quenching (qP) were decreased by Mn stress. The reduction of chlorophyll fluorescence parameters indicated that Mn stress inhibited the electronic transfer process in the plants. At high manganese concentrations (150, 200, 300 mg·L-1), ETR EQY did not vary significantly, although qP decreased progressively with each increase in Mn supply concentration to 300 mg·L-1. Changes in the pattern of plant height were similar to those of ETR and EQY, while C. argentea suffered no significant reduction in root length until the Mn concentration reached 300 mg L-1. The results indicated that C. argentea has a high tolerance to manganese.