CAJ | 학술논문

为了探明间作种植模式和接种AMF对连作土壤的影响，试验选取马铃薯连作10年土壤为基质，采用两因素随机区组盆栽实验设计，研究间作及接种AMF对连作土壤微生物群落结构及功能的影响。结果表明：在不同间作模式及AMF接种后，连作土壤微生物群落结构、功能均有不同程度的差异。间作调控后马铃薯|玉米|蚕豆处理土壤细菌比例最高，放线菌次之，真菌最低；Biolog Eco板平均颜色变化率（AWCD）比对照高36.77%。间作并接种AMF（Gloumus etunicatum）后，土壤微生物功能多样性增强，土壤真菌的比例上升，细菌、放线菌的比例下降；马铃薯|蚕豆、马铃薯|玉米|蚕豆土壤孢子密度与其他处理有显著差异（P<0.05）；马铃薯|玉米|蚕豆处理在培养96 h后的平均颜色变化率（AWCD）值比单作高48.96%。各处理土壤根际微生物群落在2种方式的调控下对碳水化合物、氨基酸碳源利用强度较高，对芳香化合物的利用能力较弱；调控后碳源利用类型开始从碳水化合物、胺类化合物向胺类化合物和多聚化合物利用类型转变。间作调控和AMF调控对土壤微生物群落功能多样性的增加可能存在一定的叠加效应。土壤微生物群落结构、功能多样性的变化是多种因子综合作用的结果。
위료탐명간작충식모식화접충AMF대련작토양적영향，시험선취마령서련작10년토양위기질，채용량인소수궤구조분재실험설계，연구간작급접충AMF대련작토양미생물군락결구급공능적영향。결과표명：재불동간작모식급AMF접충후，련작토양미생물군락결구、공능균유불동정도적차이。간작조공후마령서|옥미|잠두처리토양세균비례최고，방선균차지，진균최저；Biolog Eco판평균안색변화솔（AWCD）비대조고36.77%。간작병접충AMF（Gloumus etunicatum）후，토양미생물공능다양성증강，토양진균적비례상승，세균、방선균적비례하강；마령서|잠두、마령서|옥미|잠두토양포자밀도여기타처리유현저차이（P<0.05）；마령서|옥미|잠두처리재배양96 h후적평균안색변화솔（AWCD）치비단작고48.96%。각처리토양근제미생물군락재2충방식적조공하대탄수화합물、안기산탄원이용강도교고，대방향화합물적이용능력교약；조공후탄원이용류형개시종탄수화합물、알류화합물향알류화합물화다취화합물이용류형전변。간작조공화AMF조공대토양미생물군락공능다양성적증가가능존재일정적첩가효응。토양미생물군락결구、공능다양성적변화시다충인자종합작용적결과。
In order to comprehend the effects of intercropping and inoculation of AMF on the continuous cropping soil, we selected the soil from the south of Ningxia region which has been continuous cropping for ten years as the substace, and used the two factor randomized block pot experimental design to study the effects of intercropping and inoculation of AMF on its soil microbial community structure and function. The results showed: After taking the control of intercropping and inoculation of AMF, the microbial community structural and functional diversity had some certain differences. Under the regulation of intercropping, the percentage of bacteria in potato/maize/broad bean was the highest, actinomycetes followed, and fungus lowest; The AWCD was 36.77%higher than CK. After inoculated AMF(G. etunicatum), the microbial functional diversity increased, the percentage of fugus was increased, bacteria and attinomycetes dropped; The spore density in potato/broad bean and potato/maize/broad bean had a significant difference with other treatments(P<0.05); The AWCD in potato/maize/broad bea increased 48.96% than CK. The soil microorganisms in all treatments under this two controls had a greater usage intensity of carbon compound and amino acid, and used the aromatic compound lest ; The carbon resource utilization patter changed from carbon compound, amine compound to amine compound and polymer compound after the regulation. And maybe there's an overlapping effects between the regulation of intercropping and AMF on the improvement of the soil microbial community structural and functional diversity which was combined affected by multi-factors.