生态环境学报
生態環境學報
생태배경학보
ECOLOGY AND ENVIRONMENT
2013年
8期
1335-1340
,共6页
斜纹夜蛾%蛋白酶抑制剂%发育历期
斜紋夜蛾%蛋白酶抑製劑%髮育歷期
사문야아%단백매억제제%발육력기
Spodoptera litura%protease inhibitor%development stage
以广食性害虫斜纹夜蛾（Prodenia litura Fabriciu）为研究对象，连续6代自二龄或三龄开始用含有大豆胰蛋白酶抑制剂（soybean trypsin inhibitor，SBTI）的人工饲料饲养幼虫，饲养至乳化时分别测定SBTI对斜纹夜蛾幼虫体质量、发育历期和蛹质量的影响。结果表明，SBTI显著抑制幼虫体质量的增长，使幼虫发育延缓，连续饲养6代，与对照相比，对2龄、3龄SBTI处理的幼虫体质量的抑制率分别从60%降至21%、49%降至17%；SBTI显著抑制斜纹夜蛾的蛹质量，使蛹质量减轻，连续饲养6代，与对照相比，对2龄、3龄SBTI处理的幼虫其蛹质量的抑制率分别从37%降至4%、16%降至2%；SBTI延迟幼虫的生长发育，使得世代历期延长；而且 SBTI 对低龄幼虫的抑制作用更加显著。随着继代代数的增加，SBTI 对幼虫体质量的抑制作用减小，体质量增长加快，幼虫发育期缩短；SBTI 对蛹质量的抑制作用减弱，蛹质量增加，斜纹夜蛾世代历期缩短。研究结果说明斜纹夜蛾可以通过调节自身的生长发育过程，提高幼虫适应SBTI的能力，逐步缓解SBTI的抑制作用。
以廣食性害蟲斜紋夜蛾（Prodenia litura Fabriciu）為研究對象，連續6代自二齡或三齡開始用含有大豆胰蛋白酶抑製劑（soybean trypsin inhibitor，SBTI）的人工飼料飼養幼蟲，飼養至乳化時分彆測定SBTI對斜紋夜蛾幼蟲體質量、髮育歷期和蛹質量的影響。結果錶明，SBTI顯著抑製幼蟲體質量的增長，使幼蟲髮育延緩，連續飼養6代，與對照相比，對2齡、3齡SBTI處理的幼蟲體質量的抑製率分彆從60%降至21%、49%降至17%；SBTI顯著抑製斜紋夜蛾的蛹質量，使蛹質量減輕，連續飼養6代，與對照相比，對2齡、3齡SBTI處理的幼蟲其蛹質量的抑製率分彆從37%降至4%、16%降至2%；SBTI延遲幼蟲的生長髮育，使得世代歷期延長；而且 SBTI 對低齡幼蟲的抑製作用更加顯著。隨著繼代代數的增加，SBTI 對幼蟲體質量的抑製作用減小，體質量增長加快，幼蟲髮育期縮短；SBTI 對蛹質量的抑製作用減弱，蛹質量增加，斜紋夜蛾世代歷期縮短。研究結果說明斜紋夜蛾可以通過調節自身的生長髮育過程，提高幼蟲適應SBTI的能力，逐步緩解SBTI的抑製作用。
이엄식성해충사문야아（Prodenia litura Fabriciu）위연구대상，련속6대자이령혹삼령개시용함유대두이단백매억제제（soybean trypsin inhibitor，SBTI）적인공사료사양유충，사양지유화시분별측정SBTI대사문야아유충체질량、발육력기화용질량적영향。결과표명，SBTI현저억제유충체질량적증장，사유충발육연완，련속사양6대，여대조상비，대2령、3령SBTI처리적유충체질량적억제솔분별종60%강지21%、49%강지17%；SBTI현저억제사문야아적용질량，사용질량감경，련속사양6대，여대조상비，대2령、3령SBTI처리적유충기용질량적억제솔분별종37%강지4%、16%강지2%；SBTI연지유충적생장발육，사득세대력기연장；이차 SBTI 대저령유충적억제작용경가현저。수착계대대수적증가，SBTI 대유충체질량적억제작용감소，체질량증장가쾌，유충발육기축단；SBTI 대용질량적억제작용감약，용질량증가，사문야아세대력기축단。연구결과설명사문야아가이통과조절자신적생장발육과정，제고유충괄응SBTI적능력，축보완해SBTI적억제작용。
The larval weight gain, pupa weight, and larval development phase of Spodoptera litura were evaluated after S. litura continuous exposure to diets containing soybean trypsin inhibitor (SBTI) from the second or third instars for six generations. SBTI could significantly inhibit the growth of larval weight and delayed the development of S. litura. Compared to the control, the inhibition rate on larval weight and larval weight was declined from 60%to 21%and 37%to 4%, 49%to 17%and 16%to 2%after the 2nd instars or 3rd instars feeding with SBTI for six generations. SBTI could delay the larval growth and development stage of S. litura. SBTI showed more inhibited effect on the younger larvae. With the incensement of subculture algebra, the inhibition effect of SBTI on larval weight was decreased, the growth of weight was accelerated and larval development period become shortened. Our study indicated that S. litura could regulate their growth and development process, improves the ability of larvae to adapt to SBTI, and gradually eased the inhibition effect of SBTI.
