CAJ | 학술논문

制备高效降解亚硝酸盐的乳酸菌冻干剂，并对制剂的存活率、亚硝酸盐降解率及其产亚硝酸盐还原酶的活力影响进行研究。应用真空冷冻干燥法，对添加保护剂的3种乳酸菌分别进行菌种冻干剂的制备，用菌落计数法测定菌种存活率，分光光度法测定亚硝酸盐降解率和酶活力。结果表明，菌种制剂存活率高于51.4%；亚硝酸盐降解率与冻干前相比差异不显著；冻干剂产亚硝酸盐还原酶的活力较冻干前相比分别下降了7.62，9.02，5.44 U/mL。真空冷冻干燥乳酸菌能保持菌株原有特性。
제비고효강해아초산염적유산균동간제，병대제제적존활솔、아초산염강해솔급기산아초산염환원매적활력영향진행연구。응용진공냉동간조법，대첨가보호제적3충유산균분별진행균충동간제적제비，용균락계수법측정균충존활솔，분광광도법측정아초산염강해솔화매활력。결과표명，균충제제존활솔고우51.4%；아초산염강해솔여동간전상비차이불현저；동간제산아초산염환원매적활력교동간전상비분별하강료7.62，9.02，5.44 U/mL。진공냉동간조유산균능보지균주원유특성。
The vacuum cryodesiccation lactic acid bacteria with high efficient degradation of nitrite is obtained, and the survival rate of bacterial preparations, decomposition rate of nitrite and the effect of nitrite reductase are studied. The vacuum cryodesiccation method is used respectively to prepare the vacuum cryodesiccation strain of the three kinds of lactic acid bacteria added protective agent. Survival rate is traced by colony counting method, the nitrite degradation rate and enzyme activity are determined. The results show that the survival rate of bacterial preparation is higher than 51.4%; after treatment the change of nitrite degradation rate is not significant. Compared with before, the activity of nitrite reductase produced by the vacuum cryodesiccation strain decreases by 7.62, 9.02, 5.44 U/mL respectively. The vacuum cryodesiccation lactic acid bacteria can maintain its original characteristics.