CAJ | 학술논문

目的：观察反复正加速度(+Gz)暴露后自发性高血压大鼠(spontaneously hypertensive rats，SHR)心、脑、肾、主动脉的病理改变。方法14只8周龄SHR及14只8周龄Wistar Kyoto大鼠(wistar kyoto rats，WKY)随机分为4组，SHR+Gz暴露组，SHR对照组，正常WKY+Gz暴露组，正常WKY对照组，每组各7只。+Gz暴露采取模拟空战动作模式连续进行，依次为+5 Gz/10 s、+9 Gz/10 s、+5 Gz/10 s、+9 Gz/10 s、+5 Gz/10 s和+9 Gz/10 s，G增长率为1 G/s。休息20 min后，再按上述模式重复暴露1次，连续进行7d暴露。对照组大鼠不进行离心机暴露。所有大鼠在实验结束后立即采集心、脑、肾、主动脉标本，进行HE染色光镜观察，并对左心室心肌质量指数(left ventricle mass index，LVMI)进行测量。结果 SHR大鼠LVMI明显高于正常WKY大鼠(P＜0.01)，+Gz暴露SHR大鼠LVMI明显高于对照SHR组(P＜0.01)。光镜观察两组WKY大鼠靶器官未见明显组织学损害表现；SHR+Gz暴露组与SHR对照组大鼠存在心肌、肾脏、大脑皮质损害表现， SHR+Gz暴露组损害程度重于SHR对照组。结论+Gz暴露可以加重SHR靶器官的组织学损害程度。
목적：관찰반복정가속도(+Gz)폭로후자발성고혈압대서(spontaneously hypertensive rats，SHR)심、뇌、신、주동맥적병리개변。방법14지8주령SHR급14지8주령Wistar Kyoto대서(wistar kyoto rats，WKY)수궤분위4조，SHR+Gz폭로조，SHR대조조，정상WKY+Gz폭로조，정상WKY대조조，매조각7지。+Gz폭로채취모의공전동작모식련속진행，의차위+5 Gz/10 s、+9 Gz/10 s、+5 Gz/10 s、+9 Gz/10 s、+5 Gz/10 s화+9 Gz/10 s，G증장솔위1 G/s。휴식20 min후，재안상술모식중복폭로1차，련속진행7d폭로。대조조대서불진행리심궤폭로。소유대서재실험결속후립즉채집심、뇌、신、주동맥표본，진행HE염색광경관찰，병대좌심실심기질량지수(left ventricle mass index，LVMI)진행측량。결과 SHR대서LVMI명현고우정상WKY대서(P＜0.01)，+Gz폭로SHR대서LVMI명현고우대조SHR조(P＜0.01)。광경관찰량조WKY대서파기관미견명현조직학손해표현；SHR+Gz폭로조여SHR대조조대서존재심기、신장、대뇌피질손해표현， SHR+Gz폭로조손해정도중우SHR대조조。결론+Gz폭로가이가중SHR파기관적조직학손해정도。
Objective To observe the repeated +Gz exposure induced damage to heart, brain, kidneys and aorta in rats with spontaneous hypertension (SH). Methods Fourteen SH rats and 14 Wistar Kyoto rats (WKY) at the age of 8 weeks were randomly divided into SH+Gz exposure group,SH control group,WKY+Gz exposure group and WKY control group (7 in each group). The rats were exposed to+5 Gz/10 s,+9 Gz/10 s,+5 Gz/10 s,+9 Gz/10 s,+5 Gz/10 s and+9 Gz/10 s respectively by simulating air combat maneuver (SACM), which were repeated after the rats had a 20 min rest, total for 7 days. Samples taken from the heart, brain, kidneys and aorta of the rats at the end of exposure were stained with HE and observed under light microscope. The left ventricular mass index (LVMI) was measured. Results The LVMI was significantly higher in SH rat groups than in WKY rat groups (P< 0.01), and was significantly higher in SH+Gz exposure group than in SH control group. No significant target organ damage was observed in WKY+Gz exposure group and WKY control group under optical microscope. The damage to cardiac muscle, kidneys and cerebral cortex was severer in SH+Gz exposure group than in SH control group although it was observed both in the two groups. Conclusion +Gz exposure can aggravate the damage to target organs in SH rats.