中南大学学报（医学版）
中南大學學報（醫學版）
중남대학학보（의학판）
JOURNAL OF CENTRAL SOUTH UNIVERSITY (MEDICAL SCIENCES)
2013年
9期
944-948
,共5页
易果果%谭浅%汪澎%蒋剑%刘丹
易果果%譚淺%汪澎%蔣劍%劉丹
역과과%담천%왕팽%장검%류단
血小板反应蛋白-1%氧诱导视网膜病变%视网膜血管生长发育抑制期
血小闆反應蛋白-1%氧誘導視網膜病變%視網膜血管生長髮育抑製期
혈소판반응단백-1%양유도시망막병변%시망막혈관생장발육억제기
thrombonspondin-1%oxygen-induced retinopathy%retinal neovascularization
目的：：检测血小板反应蛋白-1(thrombonspondin-1，TSP-1)在氧诱导视网膜病变(oxygen-induced retinopathy， OIR)模型小鼠视网膜中的表达，探讨其在视网膜新生血管中的作用。方法：随机选取7日龄C57BL/6J新生小鼠40只，分为模型组(n=20)及正常对照组(n=20)。模型组小鼠通过高氧诱导的方法建立OIR模型。于小鼠出生后第7，9，11天时两组各随机抽取5只小鼠，取视网膜组织采用RT-PCR法检测TSP-1 mRNA的表达水平；并于小鼠出生后第11天时两组随机取5只小鼠，运用荧光造影视网膜铺片对视网膜新生血管进行形态学观察。结果：出生后第11天时，正常组小鼠视网膜铺片显示视网膜血管分布呈均匀的网状结构，而模型组小鼠视盘周围可见大片无灌注区，视网膜大血管扩张，仅在周边见少量毛细血管分布，为典型的OIR早期表现。出生后第7天，模型组与对照组小鼠视网膜组织中TSP-1 mRNA表达水平差异无统计学意义(P>0.05)；出生后第9天，模型组小鼠视网膜组织中TSP-1 mRNA表达水平下降(P<0.05)；出生后第11天模型组小鼠视网膜组织中TSP-1 mRNA表达水平明显低于正常组(P<0.01)，且较第9天模型组亦下降(P<0.05)。结论：在新生小鼠OIR模型视网膜血管生长发育抑制期，视网膜组织中TSP-1 mRNA的表达逐渐下降，提示TSP-1可能作为负调节因子在早期参与视网膜新生血管的形成过程。
目的：：檢測血小闆反應蛋白-1(thrombonspondin-1，TSP-1)在氧誘導視網膜病變(oxygen-induced retinopathy， OIR)模型小鼠視網膜中的錶達，探討其在視網膜新生血管中的作用。方法：隨機選取7日齡C57BL/6J新生小鼠40隻，分為模型組(n=20)及正常對照組(n=20)。模型組小鼠通過高氧誘導的方法建立OIR模型。于小鼠齣生後第7，9，11天時兩組各隨機抽取5隻小鼠，取視網膜組織採用RT-PCR法檢測TSP-1 mRNA的錶達水平；併于小鼠齣生後第11天時兩組隨機取5隻小鼠，運用熒光造影視網膜鋪片對視網膜新生血管進行形態學觀察。結果：齣生後第11天時，正常組小鼠視網膜鋪片顯示視網膜血管分佈呈均勻的網狀結構，而模型組小鼠視盤週圍可見大片無灌註區，視網膜大血管擴張，僅在週邊見少量毛細血管分佈，為典型的OIR早期錶現。齣生後第7天，模型組與對照組小鼠視網膜組織中TSP-1 mRNA錶達水平差異無統計學意義(P>0.05)；齣生後第9天，模型組小鼠視網膜組織中TSP-1 mRNA錶達水平下降(P<0.05)；齣生後第11天模型組小鼠視網膜組織中TSP-1 mRNA錶達水平明顯低于正常組(P<0.01)，且較第9天模型組亦下降(P<0.05)。結論：在新生小鼠OIR模型視網膜血管生長髮育抑製期，視網膜組織中TSP-1 mRNA的錶達逐漸下降，提示TSP-1可能作為負調節因子在早期參與視網膜新生血管的形成過程。
목적：：검측혈소판반응단백-1(thrombonspondin-1，TSP-1)재양유도시망막병변(oxygen-induced retinopathy， OIR)모형소서시망막중적표체，탐토기재시망막신생혈관중적작용。방법：수궤선취7일령C57BL/6J신생소서40지，분위모형조(n=20)급정상대조조(n=20)。모형조소서통과고양유도적방법건립OIR모형。우소서출생후제7，9，11천시량조각수궤추취5지소서，취시망막조직채용RT-PCR법검측TSP-1 mRNA적표체수평；병우소서출생후제11천시량조수궤취5지소서，운용형광조영시망막포편대시망막신생혈관진행형태학관찰。결과：출생후제11천시，정상조소서시망막포편현시시망막혈관분포정균균적망상결구，이모형조소서시반주위가견대편무관주구，시망막대혈관확장，부재주변견소량모세혈관분포，위전형적OIR조기표현。출생후제7천，모형조여대조조소서시망막조직중TSP-1 mRNA표체수평차이무통계학의의(P>0.05)；출생후제9천，모형조소서시망막조직중TSP-1 mRNA표체수평하강(P<0.05)；출생후제11천모형조소서시망막조직중TSP-1 mRNA표체수평명현저우정상조(P<0.01)，차교제9천모형조역하강(P<0.05)。결론：재신생소서OIR모형시망막혈관생장발육억제기，시망막조직중TSP-1 mRNA적표체축점하강，제시TSP-1가능작위부조절인자재조기삼여시망막신생혈관적형성과정。
Objective:To examine the expression and function of thrombonspondin-1 (TSP-1) in oxygen-induced retinopathy in new-born mice, and to investigate its role in retinal neovascularization. Methods:A total of 40 C57BL/6J newborn mice were divided equally into a model group (n=20)andanormalcontrolgroup(n=20).Miceinthemodelgroupwereexposedto75%oxygentoestablish the oxygen-induced retinopathy (OIR) model. On the 7th, 9th, and 11th day after the birth of mice, 5 mice were randomly selected each time from the 2 groups to examine the expression of TSP-1 mRNA with reverse transcription polymerase chain reaction (RT-PCR). After that, 5 mice were selected on the 11th day to observe the retinal neovascularization by fluorescein angiography retinal flatmount. Results:On the 11th day, fluorescein angiography retinal flatmount showed that the retinal blood vessels presented mean network distribution in the normal control group, while in the model group, a lot of dilatated areas in the retinal main vessels surrounded the optic disc. Meanwhile lots of new blood vessels were found surrounding the optic disc with irregular distribution but well distributed peripherial retinal small vessels, which was typical of early stage OIR. There was no signiifcant difference in the retinal TSP-1 mRNA level between the model group and the normal control group in the postnatal 7-day mice (P>0.05). Compared with the normal control group, the expression of TSP-1 mRNA in the model group was signiifcantly lower in postnatal 9-day and 11-day mice (P<0.05, P<0.01) , and the expression of TSP-1 mRNA in postnatal 9-day mice was lower than that in the postnatal 11-day mice(P<0.05). <br> Conclusion:In the early stage of OIR model (retinal vascular growth and development stage), the expression of TSP-1 mRNA in the retinal tissue is gradually decreased, implying that TSP-1 (as a negative regulatory factor) may be involved in the formation of retinal neovascularization in the early stage.
