CAJ | 학술논문

目前有多种发展微卫星标记(SSR)的方法，对基因组未测序的物种利用近缘物种引物进行跨物种扩增是一种简便方法。本研究利用马铃薯的640对SSR引物对茄子进行扩增，并利用获得的多态引物进行了12份茄子材料的聚类分析。结果表明：192对引物能够在茄子基因组DNA上扩增出清晰、稳定条带，引物通用率为30.05%；10对引物在随机选择的4份材料上有多态性，聚类分析结果表明类群与材料的来源、果实形态、颜色及青枯病抗性间没有相关趋势。
목전유다충발전미위성표기(SSR)적방법，대기인조미측서적물충이용근연물충인물진행과물충확증시일충간편방법。본연구이용마령서적640대SSR인물대가자진행확증，병이용획득적다태인물진행료12빈가자재료적취류분석。결과표명：192대인물능구재가자기인조DNA상확증출청석、은정조대，인물통용솔위30.05%；10대인물재수궤선택적4빈재료상유다태성，취류분석결과표명류군여재료적래원、과실형태、안색급청고병항성간몰유상관추세。
There are several methods of developing markers of simple sequence repeats (SSR). For those species of no genomic sequences available in database, cross-species amplification of SSRs is a simple and feasible method. 640 potato SSR primer pairs were tested on eggplants in this study to identify transferable markers and the polymorphic SSRs were further used to do the cluster analysis of twelve eggplant materials. The results showed that 192 potato SSRs could successfully amplify apparent and robust bands on eggplants, indicating a transferability rate of 30.05%. 10 SSRs were polymorphic on four randomly chosen materials. The cluster analysis indicated that there was no trend of correlation between clusters and origin, fruit shape, color and bacterial wilt resistance.