CAJ | 학술논문

目的研究比阿培南等抗生素诱导鲍曼不动杆菌释放内毒素（LPS）的作用机制。方法比阿培南、美罗培南、左氧氟沙星、头孢他啶4种药物以不同浓度作用于鲍曼不动杆菌，采用鲎试剂动态浊度法检测不同时间点的LPS含量；检测各药物在0.5×MIC时的细菌数量，并观察作用7 h后细菌的形态学改变。结果与对照组比较，各给药组释放的LPS均明显减少，且各高浓度给药组的LPS释放在作用后期趋于平缓。各给药组的细菌数量均明显低于对照组。细菌形态学研究显示，头孢他啶和左氧氟沙星组的细菌形态呈丝状，比阿培南和美罗培南组呈球形，且比阿培南组可观察到破碎菌体。结论 LPS释放水平与药物种类、药物浓度、作用时间等因素有关；比阿培南、美罗培南、左氧氟沙星、头孢他啶诱导鲍曼不动杆菌细菌释放LPS的作用机制不同。
목적연구비아배남등항생소유도포만불동간균석방내독소（LPS）적작용궤제。방법비아배남、미라배남、좌양불사성、두포타정4충약물이불동농도작용우포만불동간균，채용후시제동태탁도법검측불동시간점적LPS함량；검측각약물재0.5×MIC시적세균수량，병관찰작용7 h후세균적형태학개변。결과여대조조비교，각급약조석방적LPS균명현감소，차각고농도급약조적LPS석방재작용후기추우평완。각급약조적세균수량균명현저우대조조。세균형태학연구현시，두포타정화좌양불사성조적세균형태정사상，비아배남화미라배남조정구형，차비아배남조가관찰도파쇄균체。결론 LPS석방수평여약물충류、약물농도、작용시간등인소유관；비아배남、미라배남、좌양불사성、두포타정유도포만불동간균세균석방LPS적작용궤제불동。
Objective To study mechanisms of lipopolysaccharide (LPS) release from Acinetobacter baumannii (A. baumannii) induced by biapenem (BIPM), meropenem (MEM) and levofloxacin (LVFX). Methods A. baumannii was ex-posed to different concentrations of BIPM, MEM, LVFX, and ceftazidime (CAZ) at different time points. The LPS release was measured by tachypleus amebocyte lysate (TAL) test. Quantities of bacteria exposing to 0.5 MIC antibiotics were measured. The morphology of bacteria treated with different antibiotics for 7-hour was evaluated by light microscope. Results The LPS releases of A. baumannii treated with four kinds of antibiotics were significantly less than those of control group. In the later period, LPS releases of A. baumannii exposed to the high concentration of antibiotics tended to smooth. The cell quanti-ties of bacteria were significantly lower in treatment group than that of control group. The cells of A. baumannii treated with CAZ and LVFX showed long filamentous shape. The bacteria exposed to MEM and BIPM showed obviously globular. At the same time, some bacterial cells were induced to lysis rapidly by BIPM. Conclusion The level of LPS release is influenced by kinds of antibiotics, concentration and incubation period. All kinds of antibiotics could induce morphological changes of A. baumannii, which may be related to the different mechanisms that bacteria induce the different levels of LPS release.