天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2013年
10期
957-961
,共5页
王红芳%许力军%牛晓莉%王明霞
王紅芳%許力軍%牛曉莉%王明霞
왕홍방%허력군%우효리%왕명하
头孢他啶%β内酰胺类%氧氟沙星%鲍氏不动杆菌%内毒素类%比阿培南%美罗培南
頭孢他啶%β內酰胺類%氧氟沙星%鮑氏不動桿菌%內毒素類%比阿培南%美囉培南
두포타정%β내선알류%양불사성%포씨불동간균%내독소류%비아배남%미라배남
ceftazidime%beta-lactams%ofloxacin%acinetobacter baumannii%endotoxins%biapenem%meropenem
目的研究比阿培南等抗生素诱导鲍曼不动杆菌释放内毒素(LPS)的作用机制。方法比阿培南、美罗培南、左氧氟沙星、头孢他啶4种药物以不同浓度作用于鲍曼不动杆菌,采用鲎试剂动态浊度法检测不同时间点的LPS含量;检测各药物在0.5×MIC时的细菌数量,并观察作用7 h后细菌的形态学改变。结果与对照组比较,各给药组释放的LPS均明显减少,且各高浓度给药组的LPS释放在作用后期趋于平缓。各给药组的细菌数量均明显低于对照组。细菌形态学研究显示,头孢他啶和左氧氟沙星组的细菌形态呈丝状,比阿培南和美罗培南组呈球形,且比阿培南组可观察到破碎菌体。结论 LPS释放水平与药物种类、药物浓度、作用时间等因素有关;比阿培南、美罗培南、左氧氟沙星、头孢他啶诱导鲍曼不动杆菌细菌释放LPS的作用机制不同。
目的研究比阿培南等抗生素誘導鮑曼不動桿菌釋放內毒素(LPS)的作用機製。方法比阿培南、美囉培南、左氧氟沙星、頭孢他啶4種藥物以不同濃度作用于鮑曼不動桿菌,採用鱟試劑動態濁度法檢測不同時間點的LPS含量;檢測各藥物在0.5×MIC時的細菌數量,併觀察作用7 h後細菌的形態學改變。結果與對照組比較,各給藥組釋放的LPS均明顯減少,且各高濃度給藥組的LPS釋放在作用後期趨于平緩。各給藥組的細菌數量均明顯低于對照組。細菌形態學研究顯示,頭孢他啶和左氧氟沙星組的細菌形態呈絲狀,比阿培南和美囉培南組呈毬形,且比阿培南組可觀察到破碎菌體。結論 LPS釋放水平與藥物種類、藥物濃度、作用時間等因素有關;比阿培南、美囉培南、左氧氟沙星、頭孢他啶誘導鮑曼不動桿菌細菌釋放LPS的作用機製不同。
목적연구비아배남등항생소유도포만불동간균석방내독소(LPS)적작용궤제。방법비아배남、미라배남、좌양불사성、두포타정4충약물이불동농도작용우포만불동간균,채용후시제동태탁도법검측불동시간점적LPS함량;검측각약물재0.5×MIC시적세균수량,병관찰작용7 h후세균적형태학개변。결과여대조조비교,각급약조석방적LPS균명현감소,차각고농도급약조적LPS석방재작용후기추우평완。각급약조적세균수량균명현저우대조조。세균형태학연구현시,두포타정화좌양불사성조적세균형태정사상,비아배남화미라배남조정구형,차비아배남조가관찰도파쇄균체。결론 LPS석방수평여약물충류、약물농도、작용시간등인소유관;비아배남、미라배남、좌양불사성、두포타정유도포만불동간균세균석방LPS적작용궤제불동。
Objective To study mechanisms of lipopolysaccharide (LPS) release from Acinetobacter baumannii (A. baumannii) induced by biapenem (BIPM), meropenem (MEM) and levofloxacin (LVFX). Methods A. baumannii was ex-posed to different concentrations of BIPM, MEM, LVFX, and ceftazidime (CAZ) at different time points. The LPS release was measured by tachypleus amebocyte lysate (TAL) test. Quantities of bacteria exposing to 0.5 MIC antibiotics were measured. The morphology of bacteria treated with different antibiotics for 7-hour was evaluated by light microscope. Results The LPS releases of A. baumannii treated with four kinds of antibiotics were significantly less than those of control group. In the later period, LPS releases of A. baumannii exposed to the high concentration of antibiotics tended to smooth. The cell quanti-ties of bacteria were significantly lower in treatment group than that of control group. The cells of A. baumannii treated with CAZ and LVFX showed long filamentous shape. The bacteria exposed to MEM and BIPM showed obviously globular. At the same time, some bacterial cells were induced to lysis rapidly by BIPM. Conclusion The level of LPS release is influenced by kinds of antibiotics, concentration and incubation period. All kinds of antibiotics could induce morphological changes of A. baumannii, which may be related to the different mechanisms that bacteria induce the different levels of LPS release.