CAJ | 학술논문

目的探讨荧光原位杂交（Fluorescence in situ Hybridization，FISH）技术在哈萨克族食管鳞状细胞癌分子病理学诊断及预后判断中的临床应用价值。方法选择新疆医科大学第一附属医院行食管癌根治手术治疗的食管鳞状细胞癌患者40例和正常对照组10例，采用 FISH 技术，以手术切除新鲜癌组织为标本，检测3号、17号染色体着丝粒（CEP3、CEP17）的异常拷贝，并与传统的病理学诊断进行比较。结果40例患者的 CEP3和CEP17均出现异常拷贝即异倍体（aneuplody）。CEP3和CEP17在高、中、低分化癌组织中的变异率分别为42．0％和40．5％、62．9％和46．8％、76．95％和63．5％。CEP3和 CEP17的平均变异率分别为61．2％、61．0％。结论FISH技术在哈萨克族食管癌的诊断中可作为客观性检测指标在临床上作为传统病理诊断的辅助性诊断措施应用。CEP3和CEP17探针可能作为预测食管癌患者预后的生物指标。
목적탐토형광원위잡교（Fluorescence in situ Hybridization，FISH）기술재합살극족식관린상세포암분자병이학진단급예후판단중적림상응용개치。방법선택신강의과대학제일부속의원행식관암근치수술치료적식관린상세포암환자40례화정상대조조10례，채용 FISH 기술，이수술절제신선암조직위표본，검측3호、17호염색체착사립（CEP3、CEP17）적이상고패，병여전통적병이학진단진행비교。결과40례환자적 CEP3화CEP17균출현이상고패즉이배체（aneuplody）。CEP3화CEP17재고、중、저분화암조직중적변이솔분별위42．0％화40．5％、62．9％화46．8％、76．95％화63．5％。CEP3화 CEP17적평균변이솔분별위61．2％、61．0％。결론FISH기술재합살극족식관암적진단중가작위객관성검측지표재림상상작위전통병리진단적보조성진단조시응용。CEP3화CEP17탐침가능작위예측식관암환자예후적생물지표。
Objective To investigate the clinical applications and values of fluorescence in situ hybridization (FISH)technology in Kazakh patients’with esophageal squamous cell carcinoma in terms of molecular pathological diagnosis and prognosis.Methods 40 patients with esophageal cancer with radical surgical treatment in the department of Thoracic Surgery,First Affiliated Hospital of Xinjiang Medical University and 10 cases of normal specimen in control group were enrolled in this study.Fresh cancer tissues were made to analyze the copy number of centromere in chromosome 3 and 1 7 abnormalities by comparing with routine pathological diagnosis.Results FISH analysis revealed that abnormal copy numbers were observed in 40 cases in either chromosome 3 and 17,manifested as chromosome abnormality(polyploidy).The polyploidy rates were 42%,40.5% in well-differentiated cancer,62.9%,46.8% in moderately differentia-ted cancer,and 76.95%,63.5% in poorly differentiated squamous cell carcer respectively.The average mutation rate of CEP3 and CEP17 were 61.2%,50.95%.Conclusion As an objective and qualitative method,the technology of FISH can detect chromosome 3 and 1 7 variations in the diagnosis of Kazakh e-sophageal cancer and can be used as a complementary method in the pathological diagnosis of esophageal cancer.In addition,CEP3 and CEP1 7 probe can be a predictive biological marker in the prognosis of pa-tients with esophageal cancer.