CAJ | 학술논문

为探索猪细小病毒M2株在ST细胞中培养的适宜条件，将其分别以同步接毒法和单层接毒法接种于ST细胞，比较其接种效果，选择更有效的方法，再对其培养条件（细胞浓度、血清种类、血清含量和收毒时间等）进行优化。结果表明，同步接种法相对于单层接种法更具优势。同步接毒，采用含量为5%的胎牛血清或6%的新生牛血清培养，细胞浓度控制在3.2×104～1.5×105个/mL内，在病毒接种后，80%以上细胞出现细胞病变时收获病毒，能获得较高病毒含量的病毒液。本研究结果为制备高效价的PPV- M2株病毒抗原提供了数据资料。
위탐색저세소병독M2주재ST세포중배양적괄의조건，장기분별이동보접독법화단층접독법접충우ST세포，비교기접충효과，선택경유효적방법，재대기배양조건（세포농도、혈청충류、혈청함량화수독시간등）진행우화。결과표명，동보접충법상대우단층접충법경구우세。동보접독，채용함량위5%적태우혈청혹6%적신생우혈청배양，세포농도공제재3.2×104～1.5×105개/mL내，재병독접충후，80%이상세포출현세포병변시수획병독，능획득교고병독함량적병독액。본연구결과위제비고효개적PPV- M2주병독항원제공료수거자료。
To explore the suitable condition for culturing Porcine Parvovirus M2 Strain in ST Cells, synchronous inoculation and single-layer vaccination were be taken to culturing PPV-M2 strain. Then, the more effective method was choosed to optimize the culturing condition of PPV-M2 Strain in ST cells. Results showed that the method of synchronous inoculation has more advantages compared with sin-gle-layer inoculation method for culturing PPV M2 Strain. And The PPV M2 strain would propagate effectively in ST cells when controlling the density in 3.2 × 104~1.5×105/mL,using DMEM with 5% fetal bovine serum or 6% new-born calf serum as cul-ture medium,harvest the virus within 80% of the cells appearing CPE. This research could give data support for the preparation of high titer PPV antigen of M2 Strain.