CAJ | 학술논문

本研究建立了测定生鲜乳中己烯雌酚（diethylstilbestrol）、雌二醇（estradiol）、炔雌醇（ethi-nylestradiol）、戊酸雌二醇（estradiol valerate）残留量的GC- MS法，为控制食源性动物食品的质量安全提供依据。本试验的样品经乙腈和乙酸乙酯提取，HLB固相萃取小柱净化，70℃衍生后在EI源SIM模式下进样分析。试验结果显示，己烯雌酚、雌二醇、炔雌醇含量在2.0~100.0μg/kg内、戊酸雌二醇含量在4.0~200.0μg/kg内线性关系良好，己烯雌酚、雌二醇、炔雌醇、戊酸雌二醇相关系数分别为0.9991、0.9987、0.9978、0.9983，平均回收率（n=6）为74.2%~85.8%（RSD为1.9%~9.2%）。该方法能够为测定生鲜乳中这4种雌激素类药物残留量提供可靠依据。
본연구건립료측정생선유중기희자분（diethylstilbestrol）、자이순（estradiol）、결자순（ethi-nylestradiol）、무산자이순（estradiol valerate）잔류량적GC- MS법，위공제식원성동물식품적질량안전제공의거。본시험적양품경을정화을산을지제취，HLB고상췌취소주정화，70℃연생후재EI원SIM모식하진양분석。시험결과현시，기희자분、자이순、결자순함량재2.0~100.0μg/kg내、무산자이순함량재4.0~200.0μg/kg내선성관계량호，기희자분、자이순、결자순、무산자이순상관계수분별위0.9991、0.9987、0.9978、0.9983，평균회수솔（n=6）위74.2%~85.8%（RSD위1.9%~9.2%）。해방법능구위측정생선유중저4충자격소류약물잔류량제공가고의거。
This research to develop and validate a rapid sensitive and specific meth-od for quantitative analytes of estrogens residues(DES, Diethylstilbestrol: E2, Es-tradiol: EE2, Ethynylestradiol: EV, Estradiol Valerate)in milk by gas chromatograph-ic-tandem mass spectrometry and be applied to estrogens residues in milk studies. The 4 kinds of components were extracted from homogenized samples with ethyl acetate fol-lowed by solid-phase extraction with HLB column and trimethylsilylation at 70 ℃ for 40 minutes, then the GC-MS method in selected ion monitoring mode(SIM)was used to an-alyze them. A good linearity was achieved with the coefficient r2 > 0.997 8(n =2). The average recovery of estrogens was more than 74.2% with RSD lower than 9.2%(n = 9). The method is sensitive, accurate, rapid, and suitable for the analysis estrogens resi-dues in milk.