CAJ | 학술논문

本研究应用分子生物学技术，扩增并测定了2011～2012年辽宁地区6株PRRSV NSP2基因全序列。结果发现，6株PRRSV的NSP2基因全长均为2850 bp，均于1143～1441 bp和1593～1689 bp发生连续的碱基缺失，且均为变异毒株，具有典型HP- PRRSV的分子特征；同源性与进化分析表明，HP- PRRS在辽宁地区有不同的毒株同时流行，辽宁地区HP- PRRSV可能起源于同一祖先。
본연구응용분자생물학기술，확증병측정료2011～2012년료녕지구6주PRRSV NSP2기인전서렬。결과발현，6주PRRSV적NSP2기인전장균위2850 bp，균우1143～1441 bp화1593～1689 bp발생련속적감기결실，차균위변이독주，구유전형HP- PRRSV적분자특정；동원성여진화분석표명，HP- PRRS재료녕지구유불동적독주동시류행，료녕지구HP- PRRSV가능기원우동일조선。
In this study, molecular biology techniques were used to amplify and se-quencing six strains PRRSV NSP2 genome sequences in swine herds in Liaoning Prov-ince from 2011 to 2012 years. It was found that full-length of six strains PRRSV NSP2 genome sequences were all 2 850 bp. continuous base deletion occurred at 1 441bp~1 143bp and 1 593bp~1 689bp of the six strains PRRSV NSP2 genome;and they were all variant strains. All of he six strains PRRSV NSP2 genome had typical HP-PRRSV molecular characteristics.The analysis of homology and phylogenetic showed that different strains of HP-PRRSV were prevalent in in swine herds in Liaon-ing Province at the same time. The strains of HP-PRRSV may originate from the same ancestor PRRSV strain in swine herds in Liaoning Province.