CAJ | 학술논문

目的：观察骨髓基质细胞(bone marrow stromal cells，BMSCs)移植对大鼠脊髓损伤后环氧化酶-2(cyclooxygenase-2， COX-2)和血管内皮生长因子(vascular endothelial growth factor，VEGF)蛋白表达的影响。方法大鼠随机分为3组：A为假手术组，B为细胞培养基(DMEM)对照组，C为BMSCs移植组。B组和C组大鼠进行脊髓压迫损伤模型制作。脊髓损伤后， B组和C组大鼠分别给予DMEM培养液或BMSCs脊髓损伤周围区注射。术后应用Western Blot方法检测COX-2、VEGF蛋白在损伤脊髓组织中的表达变化。结果 COX-2、VEGF蛋白在A组大鼠未损伤脊髓组织中均低水平表达。B组与A组相比，脊髓损伤后COX-2蛋白表达水平明显增加(P＜0.01)，VEGF蛋白表达水平则短暂显著增加(P＜0.05)。C组与B组相比， COX-2和VEGF蛋白表达水平均显著增加。结论 BMSCs移植能够增加脊髓损伤后COX-2、VEGF蛋白的表达。
목적：관찰골수기질세포(bone marrow stromal cells，BMSCs)이식대대서척수손상후배양화매-2(cyclooxygenase-2， COX-2)화혈관내피생장인자(vascular endothelial growth factor，VEGF)단백표체적영향。방법대서수궤분위3조：A위가수술조，B위세포배양기(DMEM)대조조，C위BMSCs이식조。B조화C조대서진행척수압박손상모형제작。척수손상후， B조화C조대서분별급여DMEM배양액혹BMSCs척수손상주위구주사。술후응용Western Blot방법검측COX-2、VEGF단백재손상척수조직중적표체변화。결과 COX-2、VEGF단백재A조대서미손상척수조직중균저수평표체。B조여A조상비，척수손상후COX-2단백표체수평명현증가(P＜0.01)，VEGF단백표체수평칙단잠현저증가(P＜0.05)。C조여B조상비， COX-2화VEGF단백표체수평균현저증가。결론 BMSCs이식능구증가척수손상후COX-2、VEGF단백적표체。
Objective To observe the effect of bone marrow stromal cells (BMSCs) transplantation on expression of cyclooxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF) protein in rats following spinal cord injury (SCI). Methods Adult rats were randomly divided into group A (sham operation group), group B (DMEM control group), and group C (BMSCs transplantation group). A rat spinal cord compression injury model was established. Rats in groups B and C were injected with BMSCs or DMEM after SCI. Expression of COX-2 and VEGF protein in rats following SCI was detected by Western blot. Results The expression level of COX-2 and VEGF protein was low in group A. The expression level of COX-2 and VEGF protein was significantly higher in group B than in group A and in group C than in group B (P<0.01, P<0.05). Conclusion BMSCs transplantation can up-regulate the expression of COX-2 and VEGF protein in rats following SCI.