CAJ | 학술논문

目的通过鸡松果体切除制作脊柱侧凸症的动物模型,并进行形态学观察.方法随机将Broiler鸡60只分为两组,对照组20只和松果体切除组40只.松果体切除组于出生后3d行松果体切除.3周后拍摄前后位脊柱X线片,观察脊柱曲度的改变.将鸡脊柱周围的肌肉去掉,保留脊柱,用10％福马林固定,常规苏木素-伊红(HE)染色.结果 3周时,40只行松果体切除的鸡中有25只发生了侧凸,发生率为62.5％,Cobb角为11°～25°,平均18..侧凸主要累及T6、T7和T83个椎体.通过对鸡的大体标本观察,鸡存在8对肋骨和8个胸椎,但是只有第7胸椎与相邻椎体之间形成关节样间隙,其余椎体之间呈紧密联结.结论鸡松果体切除能够制作出脊柱侧凸模型,第7胸椎与相邻椎体之间连接的结构特点有可能是鸡成为脊柱侧凸有效模型的因素之一.
목적 통과계송과체절제제작척주측철증적동물모형,병진행형태학관찰.방법 수궤장Broiler계60지분위량조,대조조20지화송과체절제조40지.송과체절제조우출생후3d행송과체절제.3주후박섭전후위척주X선편,관찰척주곡도적개변.장계척주주위적기육거도,보류척주,용10％복마림고정,상규소목소-이홍(HE)염색.결과 3주시,40지행송과체절제적계중유25지발생료측철,발생솔위62.5％,Cobb각위11°～25°,평균18..측철주요루급T6、T7화T83개추체.통과대계적대체표본관찰,계존재8대륵골화8개흉추,단시지유제7흉추여상린추체지간형성관절양간극,기여추체지간정긴밀련결.결론 계송과체절제능구제작출척주측철모형,제7흉추여상린추체지간련접적결구특점유가능시계성위척주측철유효모형적인소지일.
Objective To build the scoliosis animal model by pinealectomized chickens and have the histological observation.Methods Sixty Broiler chickens were divided into 2 groups:the control group (n =20) and the pinealectomy group (n =40).At the age of 3 days after hatching,all the chickens in the pinealectomy groups were subjected to surgery.At the 3 weeks after hatching,the chickens were sacrificed with an overdose of pentobarbital sodium.Posteranterior radiographs of the spine were taken to detect spinal curvature,and their spines were immersed in 10％ neutral phosphate-buffered formalin and have the hematoxylin and eosin (HE) staining.Results At the 3 weeks after hatching,Scoliosis (defined by a Cobb angle of more than 10°) had developed in 25 of 40 pinealectomized chickens (62.5％,average Cobb angle 18°).The chickens had eight ribs and eight thoracic vertebrae.The anatomic characteristics include no marked intervertebral discs between the third to sixth thoracic vertebrae and the eighth thoracic to the lumbosacral vertebrae,and only two intervertebral spaces between the sixth/seventh and seventh/eighth vertebrae can therefore be recognized as mobile segments.Conclusion The scoliosis animal model could be achieved by pinealectomized chickens.The structure of the seventh vertebrae may play an important role in this animal model.