中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
5期
929-931
,共3页
薛金秋%梁秀清%成琳%石靓%王莹%丁强
薛金鞦%樑秀清%成琳%石靚%王瑩%丁彊
설금추%량수청%성림%석정%왕형%정강
乳腺癌%RNA结合蛋白38%增殖%侵袭
乳腺癌%RNA結閤蛋白38%增殖%侵襲
유선암%RNA결합단백38%증식%침습
Breast cancer%RNA binding motif protein 38%Proliferation%Invasion
目的 观察RNA结合蛋白38(RNPC1)基因过表达对人乳腺癌细胞BT474的生物学特性的影响.方法 细胞分为空白对照组、阴性对照组和实验组,采用慢病毒转染法,利用细胞计数试剂盒(CCK-8)法、平板克隆形成实验、Transwell小室实验观察其对BT474细胞生物学特性的影响.结果 CCK-8实验显示,空白对照组、阴性对照组和实验组6d时吸光度(A)值分别为0.7147 ±0.0189、0.770 8±0.016 0、0.5568 ±0.043 6(P ＜0.05).克隆形成实验显示,14 d时细胞克隆形成数分别为(113.300 ±3.528)、(118.700 ±2.404)、(47.330±2.906)个(P＜0.01).Transwell小室实验显示,实验组细胞24h迁移、侵袭实验中滤过膜细胞数分别为(61.670 ±4.842)、(14.670±1.856)个,均比空白对照组和阴性对照组降低(P＜0.01).Western blot法显示,过表达RNPC1a下调基质金属蛋白酶-2(MMP-2)的蛋白表达(P＜0.01).结论 RNPC1a基因可以抑制BT474细胞的生物学特性.RNPC1a与MMP-2的相互作用关系在乳腺癌的侵袭和转移中起调节作用.
目的 觀察RNA結閤蛋白38(RNPC1)基因過錶達對人乳腺癌細胞BT474的生物學特性的影響.方法 細胞分為空白對照組、陰性對照組和實驗組,採用慢病毒轉染法,利用細胞計數試劑盒(CCK-8)法、平闆剋隆形成實驗、Transwell小室實驗觀察其對BT474細胞生物學特性的影響.結果 CCK-8實驗顯示,空白對照組、陰性對照組和實驗組6d時吸光度(A)值分彆為0.7147 ±0.0189、0.770 8±0.016 0、0.5568 ±0.043 6(P ＜0.05).剋隆形成實驗顯示,14 d時細胞剋隆形成數分彆為(113.300 ±3.528)、(118.700 ±2.404)、(47.330±2.906)箇(P＜0.01).Transwell小室實驗顯示,實驗組細胞24h遷移、侵襲實驗中濾過膜細胞數分彆為(61.670 ±4.842)、(14.670±1.856)箇,均比空白對照組和陰性對照組降低(P＜0.01).Western blot法顯示,過錶達RNPC1a下調基質金屬蛋白酶-2(MMP-2)的蛋白錶達(P＜0.01).結論 RNPC1a基因可以抑製BT474細胞的生物學特性.RNPC1a與MMP-2的相互作用關繫在乳腺癌的侵襲和轉移中起調節作用.
목적 관찰RNA결합단백38(RNPC1)기인과표체대인유선암세포BT474적생물학특성적영향.방법 세포분위공백대조조、음성대조조화실험조,채용만병독전염법,이용세포계수시제합(CCK-8)법、평판극륭형성실험、Transwell소실실험관찰기대BT474세포생물학특성적영향.결과 CCK-8실험현시,공백대조조、음성대조조화실험조6d시흡광도(A)치분별위0.7147 ±0.0189、0.770 8±0.016 0、0.5568 ±0.043 6(P ＜0.05).극륭형성실험현시,14 d시세포극륭형성수분별위(113.300 ±3.528)、(118.700 ±2.404)、(47.330±2.906)개(P＜0.01).Transwell소실실험현시,실험조세포24h천이、침습실험중려과막세포수분별위(61.670 ±4.842)、(14.670±1.856)개,균비공백대조조화음성대조조강저(P＜0.01).Western blot법현시,과표체RNPC1a하조기질금속단백매-2(MMP-2)적단백표체(P＜0.01).결론 RNPC1a기인가이억제BT474세포적생물학특성.RNPC1a여MMP-2적상호작용관계재유선암적침습화전이중기조절작용.
Objective To investigate the effect of RNA binding motif protein 38 (RNPC1) over-expression on the biological behavior of a human breast cancer cell line BT474.Methods Cell proliferation was tested by cell counting kit 8 (CCK-8) assay.The ability of colony formation was tested by plate clone formation assay.The abilities of migration and invasion were evaluated by Transwell chambers.The relative expression of matrix metalloproteinase-2 (MMP-2) protein was detected by Western blotting.Results CCK-8 test showed that the absorbance (A) value in experimental group,blank group and negative group at6th day was (0.714 7 ±0.018 9),(0.770 8 ±0.016 0) and (0.556 8 ±0.043 6),respective-ly (P ＜ 0.05).The colony formation number at 14th day was significantly less in experimental group (113.300 ±3.528) than in blank group (118.700 ±2.404) and negative group (47.330 ±2.906) (P ＜0.01).The Transwell assay revealed that the number of cells passing through the Transwell membrance in experimental group [(61.670 ±4.842) for migration,and (14.670 ± 1.856) for invation] was significantly less than in blank group and control group (both P ＜ 0.01).Overexpression of RNPC1 a down-regu-lated the expression of MMP-2 protein.Conclusion RNPC1a exhibits multiple anticancer functions in breast cancer cells.RNPC1a interacts with MMP-2 to play an important role in the metastasis of breast cancer.
