CAJ | 학술논문

目的：制备柞蚕丝素蛋白/羟基磷灰石复合支架，改善单纯羟基磷灰石(HA)支架材料的生物学特性。方法：将羟基磷灰石多孔支架材料浸泡在柞蚕丝素蛋白的水溶液中，抽真空处理48h，采取冷冻干燥法制备的方法，进行羟基磷灰石支架表面修饰。实验分为HA 支架组(单纯羟基磷灰石支架组)与（TSF/HA）柞蚕丝素蛋白/羟基磷灰石支架组。按ISO 10993标准，两组分别做体外降解实验、吸水测试及细胞粘附实验。结果：SEM镜下观察TSF/HA支架孔隙内壁上细胞粘附高于单纯HA支架，吸水率实验检测TSF-HA支架组吸水率高于单纯HA支架组，体外降解实验TSF/HA支架降解速率快于单纯HA支架，各项检测差异均有统计学意义(P<0.05)。结论： TSF/HA支架材料具有比单纯HA支架材料更优良的生物学特性,可以用于后续的实验研究。
목적：제비작잠사소단백/간기린회석복합지가，개선단순간기린회석(HA)지가재료적생물학특성。방법：장간기린회석다공지가재료침포재작잠사소단백적수용액중，추진공처리48h，채취냉동간조법제비적방법，진행간기린회석지가표면수식。실험분위HA 지가조(단순간기린회석지가조)여（TSF/HA）작잠사소단백/간기린회석지가조。안ISO 10993표준，량조분별주체외강해실험、흡수측시급세포점부실험。결과：SEM경하관찰TSF/HA지가공극내벽상세포점부고우단순HA지가，흡수솔실험검측TSF-HA지가조흡수솔고우단순HA지가조，체외강해실험TSF/HA지가강해속솔쾌우단순HA지가，각항검측차이균유통계학의의(P<0.05)。결론： TSF/HA지가재료구유비단순HA지가재료경우량적생물학특성,가이용우후속적실험연구。
Objective:Prepare the artificial scaffold with tussan silk fibroin(TSF) and hydroxyapatite(HA), improve the biological properties of simple hydroxyapatite. Methods:The porous scaffolds were soaked in TSF water solution 24hours, then these scaffolds were vacuum treatment at-10℃for 48 hours and the complex scaffold----TSF/HA were gained. The scaffolds were separated into two groups, simple HA and complex TSF/HA. According to the ISO 10993 criterion,the biological properties detection included degradation in vitro, water absorption test and celladhesion test. Result:The complex TSF/HA scaffold had innumerable TSF bal on the inner wal of cells of HA under SEM. The degradation and water absorption of complex TSF/HA scaffold were obvious fast than that of simple HA (P<0.05). The celladhesion of complex TSF/HA were significantly higher than simple HA scaffold (P<0.05). conclusion:Complex TSF/HA scaffold had bet er biological properties than HA, which could be used in the subsequent experimental studies.