CAJ | 학술논문

目的：研究大孔吸附树脂纯化银杏总黄酮的工艺条件及参数。方法：采用静态吸附、解吸，从6种大孔树脂中筛选用于银杏总黄酮分离的最佳树脂，并通过动态行为系统考察最佳大孔树脂的吸附性能和最优洗脱参数。结果：HPD-450型大孔树脂为分离银杏黄酮的最佳树脂，其分离的最佳工艺为，上样量为6.5倍柱体积药液，以5mL/min 的流速，pH 值为4.0上柱，用400mL85%乙醇以4mL/min 流速进行洗脱，结论：按照最佳工艺可获得总黄酮纯度达26%以上的银杏叶提取物。
목적：연구대공흡부수지순화은행총황동적공예조건급삼수。방법：채용정태흡부、해흡，종6충대공수지중사선용우은행총황동분리적최가수지，병통과동태행위계통고찰최가대공수지적흡부성능화최우세탈삼수。결과：HPD-450형대공수지위분리은행황동적최가수지，기분리적최가공예위，상양량위6.5배주체적약액，이5mL/min 적류속，pH 치위4.0상주，용400mL85%을순이4mL/min 류속진행세탈，결론：안조최가공예가획득총황동순도체26%이상적은행협제취물。
OBJECTIVE:To study process conditions and parameters of purification of total gingko flavones-glycoides by macroporous adsorptive resin. METHODS:To select the optimal resin used to separate glycoides among 6 different macroporous resins by static adsorption and desorption and investigate the adsorption property and optimal elution parameters of the best resin by dynamic adsorption. RESUTS:The results show that HPD-450 macroporous resin is the best resin used for separating glycosides,and the optimal technological conditions were as fol ows: the sample amount 6.5 times the column volumes, sample flow velocity 5 mL/min, pH value 4.0, eluting with 400 mL of 85% ethanol at the eluting velocity of 4mL/min. CONCLUSIONS:It turned out that total flavonoids purity more than 26% of ginkgo biloba extract according to the optimum.