CAJ | 학술논문

目的：以透射电镜观察不同抗痨药物对SD大鼠肝脏超微结构的影响.方法：健康成年SD大鼠(清洁级)152只,体重250~300g,雌雄各半,随机分为8组.观察组：H组：异烟肼(INH H)；R组：：利福平(RFP R)；Z组：吡嗪酰胺(PZA Z)；HR组：异烟肼+利福平；HZ组：异烟肼+吡嗪酰胺；RZ 组：利福平+吡嗪酰胺；HRZ 组：异烟肼+利福平+吡嗪酰胺.对照组：生理盐水.大鼠用药剂量为人每公斤体重常用剂量的5.5倍.标本制备：每天对各组大鼠进行一次灌胃,10天后处死大鼠,分离肝脏,戊二醛固定后做电镜观察.结果：肝细胞部分线粒体溶解,粗面内质网脱颗粒,滑面内质网池扩张,细胞内脂肪颗粒增多,糖原颗粒显著增多；细胞核周水肿；肝细胞间胶原纤维增生.结论：异烟肼、利福平、吡嗪酰胺均可引起大鼠肝脏的损害,多种药物联用比单药应用损害更明显；随着药物应用种类的增多,肝脏损害逐渐加重；吡嗪酰胺组比其他单用药组肝脏毒性更大.
목적：이투사전경관찰불동항로약물대SD대서간장초미결구적영향.방법：건강성년SD대서(청길급)152지,체중250~300g,자웅각반,수궤분위8조.관찰조：H조：이연정(INH H)；R조：：리복평(RFP R)；Z조：필진선알(PZA Z)；HR조：이연정+리복평；HZ조：이연정+필진선알；RZ 조：리복평+필진선알；HRZ 조：이연정+리복평+필진선알.대조조：생리염수.대서용약제량위인매공근체중상용제량적5.5배.표본제비：매천대각조대서진행일차관위,10천후처사대서,분리간장,무이철고정후주전경관찰.결과：간세포부분선립체용해,조면내질망탈과립,활면내질망지확장,세포내지방과립증다,당원과립현저증다；세포핵주수종；간세포간효원섬유증생.결론：이연정、리복평、필진선알균가인기대서간장적손해,다충약물련용비단약응용손해경명현；수착약물응용충류적증다,간장손해축점가중；필진선알조비기타단용약조간장독성경대.
Objective:To study the ultrastructure of hepatic lesion induced by antitubercular drugs. Methods:152 healthy adult rats, weighing about 250~300g,half male and half female, were randomly divided into 8 groups and given different drugs. Control group:NS;group H:isoniazid(INH H);group R:rifampicin (RFP R);group Z:pyrazinamide (PZA Z);group HR:INH+RFP;group HZ:INH+PZA;group RZ:RFP+PZA;group HRZ: INH+RFP+PZA. Rats were given 5.5 times adult clinic therapeutic dose by intragastria administration,once per day,for 10 days.The degrees of hepatic lesion were measured by means of pathology, and the effects of INH, RFP and PZA on hepatic tissues were observed. Results:Some mitochondrions in the liver cells dissolve,rough surfaced endoplasmic reticulum degranulation,smooth surfaced endoplasmic reticulum pool dilatation,the fat particles in the cell increase,hepatin particle apparently increase, dropsy appear around the karyons;collagenous fiber between the liver cells hyperplasia. Conclusion:INH, RFP and PZA can lead to the damages of hepatic tissue, especially in the usage of multiple drugs;the degree of hepatic injury is increased with the numbers of drugs used;the toxicity of PZA on liver is greater than that of other antitubercular drugs.