中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2014年
2期
138-142
,共5页
受体,血小板源生长因子%冠状动脉再狭窄%多态性单核苷酸
受體,血小闆源生長因子%冠狀動脈再狹窄%多態性單覈苷痠
수체,혈소판원생장인자%관상동맥재협착%다태성단핵감산
Receptors,platelet-derived growth factor%Coronary restenosis%Polymorphism,single nucleotide
目的 探讨血小板衍生生长因子(platelet-derived growth factor,PDGF)-B血浆水平及PDGF-B基因rs1800818、rs1800817单核苷酸多态性(single nucleotide polymorphism,SNP)与支架内再狭窄(in-stent restenosis,ISR)发生的关系. 方法 157例支架术后复查冠状动脉造影患者,分为再狭窄组(ISR组,74例)和无再狭窄组(NISR组,83例).酶联免疫吸附法(ELISA)检测血浆PDGF-B生物活性形式PDGF-BB水平、探针法实时定量聚合酶链式反应(PCR)检测PDGF-B基因型. 结果 ISR组与NISR组rs1800818 AA、AG、GG基因型频率差异无统计学意义(x2=4.48,P＞0.05),ISR组A等位基因频率高于NISR组(0.446比0.319,x2=5.33,P＜0.05).未检出rs1800817 CC基因型,两组间AA、AC基因型频率差异无统计学意义(x2=0.06,P＞0.05),A、C等位基频率差异无统计学意义(x2=0.06,P＞0.05).血浆PDGF-BB水平ISR组高于NISR组(6.53±3.65) ng/L与(5.07±2.45)ng/L(t=2.92,P＜0.01).两组间rs1800818AA基因型PDGF-BB水平分别为(9.94±4.60)ng/L与(5.90±2.98)ng/L,差异有统计学意义(t=2.69,P＜0.05).Logistic回归分析示血浆PDGF-B水平(OR=1.187,95％ CI:1.054～1.337,P＜0.01)是ISR发生的危险因素. 结论 高血浆PDGF-B水平是ISR的危险因素,PDGF-B基因SNP rs1800818、rs1800817与ISR发生无关.
目的 探討血小闆衍生生長因子(platelet-derived growth factor,PDGF)-B血漿水平及PDGF-B基因rs1800818、rs1800817單覈苷痠多態性(single nucleotide polymorphism,SNP)與支架內再狹窄(in-stent restenosis,ISR)髮生的關繫. 方法 157例支架術後複查冠狀動脈造影患者,分為再狹窄組(ISR組,74例)和無再狹窄組(NISR組,83例).酶聯免疫吸附法(ELISA)檢測血漿PDGF-B生物活性形式PDGF-BB水平、探針法實時定量聚閤酶鏈式反應(PCR)檢測PDGF-B基因型. 結果 ISR組與NISR組rs1800818 AA、AG、GG基因型頻率差異無統計學意義(x2=4.48,P＞0.05),ISR組A等位基因頻率高于NISR組(0.446比0.319,x2=5.33,P＜0.05).未檢齣rs1800817 CC基因型,兩組間AA、AC基因型頻率差異無統計學意義(x2=0.06,P＞0.05),A、C等位基頻率差異無統計學意義(x2=0.06,P＞0.05).血漿PDGF-BB水平ISR組高于NISR組(6.53±3.65) ng/L與(5.07±2.45)ng/L(t=2.92,P＜0.01).兩組間rs1800818AA基因型PDGF-BB水平分彆為(9.94±4.60)ng/L與(5.90±2.98)ng/L,差異有統計學意義(t=2.69,P＜0.05).Logistic迴歸分析示血漿PDGF-B水平(OR=1.187,95％ CI:1.054～1.337,P＜0.01)是ISR髮生的危險因素. 結論 高血漿PDGF-B水平是ISR的危險因素,PDGF-B基因SNP rs1800818、rs1800817與ISR髮生無關.
목적 탐토혈소판연생생장인자(platelet-derived growth factor,PDGF)-B혈장수평급PDGF-B기인rs1800818、rs1800817단핵감산다태성(single nucleotide polymorphism,SNP)여지가내재협착(in-stent restenosis,ISR)발생적관계. 방법 157례지가술후복사관상동맥조영환자,분위재협착조(ISR조,74례)화무재협착조(NISR조,83례).매련면역흡부법(ELISA)검측혈장PDGF-B생물활성형식PDGF-BB수평、탐침법실시정량취합매련식반응(PCR)검측PDGF-B기인형. 결과 ISR조여NISR조rs1800818 AA、AG、GG기인형빈솔차이무통계학의의(x2=4.48,P＞0.05),ISR조A등위기인빈솔고우NISR조(0.446비0.319,x2=5.33,P＜0.05).미검출rs1800817 CC기인형,량조간AA、AC기인형빈솔차이무통계학의의(x2=0.06,P＞0.05),A、C등위기빈솔차이무통계학의의(x2=0.06,P＞0.05).혈장PDGF-BB수평ISR조고우NISR조(6.53±3.65) ng/L여(5.07±2.45)ng/L(t=2.92,P＜0.01).량조간rs1800818AA기인형PDGF-BB수평분별위(9.94±4.60)ng/L여(5.90±2.98)ng/L,차이유통계학의의(t=2.69,P＜0.05).Logistic회귀분석시혈장PDGF-B수평(OR=1.187,95％ CI:1.054～1.337,P＜0.01)시ISR발생적위험인소. 결론 고혈장PDGF-B수평시ISR적위험인소,PDGF-B기인SNP rs1800818、rs1800817여ISR발생무관.
Objective To investigate the association of platelet-derived growth factor(PDGF)-B gene single nucleotide polymorphism(SNP) and plasma PDGF-BB level with in-stent restenosis(ISR) in elderly patients.Methods 157 patients who had undergone coronary artery stenting for more than half year were divided into ISR group(n=74) and NISR group(non-ISR,n=83) according to the angiographic diagnosis of in-stent restenosis (ISR).Plasma level of PDGF-BB was measured by enzyme-linked immunosorbent assay(ELISA).DNA was isolated from leukocytes.Two SNPs of the PDGF-B gene(rs1800818 and rs1800817) were determined by Taqman Quantitative Real-Time PCR with TaqMan-MGB probe.Results There were no significant differences in genotype frequency of rs1800818 AA,AG,GG between ISR group and NISR group(x2 =4.48,P＞0.05).The frequency of rs1800818A allele was much higher in ISR group than in NISR group(x2 =5.33,P＜0.05).There were no significant differences in genotype frequency of SNP rs1800817 AA and AC(x2 =0.06,P＞ 0.05) and allele frequency of SNP rs1800817 A and C(x2 =0.06,P＞0.05) between ISR group and NISR group,while genotype CC was not found.The plasma level of PDGF-BB was higher in ISR group than in NISR group [(6.53±3.65) ng/L vs.(5.07±2.45) ng/L,t=2.92,P＜0.01].Plasma level of PDGF-BB in patients with rs1800818 AA genotype was significantly higher in ISR group than in NISR group [(9.94 ± 4.60) ng/L vs.(5.90 ± 2.98) ng/L,t =2.69,P＜0.05].Multivariable logistic regression analysis showed that plasma PDGF-BB level was the risk factor for ISR (OR =1.187,95.0％ CI:1.054 1.337,P＜0.01).Conclusions High plasma PDGF-BB level is the risk factor for ISR,but PDGF-B gene SNPs rs1800818 and rs1800817 are not associated with the occurrence of ISR.
