CAJ | 학술논문

目的：通过测定18F-氟代葡萄糖（FDG）和18F-氟代胸苷（FLT）细胞结合率的变化来评估不同浓度氟尿嘧啶（5-Fu）对肺腺癌A-549细胞化疗早期反应的疗效并对两种显像剂进行初步比较。方法不同浓度（0.125、0.25、0.5、1.0 mg/mL）5-Fu 1.0 mL分别与肺腺癌A-549细胞共同孵育24 h、48 h，测定细胞对18F-FDG和18F-FLT的细胞结合变化并与MTT法测定的存活细胞数目和细胞抑制率比较。结果(1)5-Fu作用24 h和48 h后，18F-FDG和18F-FLT细胞结合率均随药物浓度的增加而下降，两者与药物浓度均呈负相关（24 h：18F-FLT r=-0.950，P＜0.01，18F-FDG r=-0.844，P＜0.01；48 h：18F-FLT r=-0.939，P＜0.01，18F-FDG r=-0.786，P＜0.01）；48 h与24 h相比，两种显像剂细胞摄取率均降低；(2)24 h和48 h MTT细胞抑制率与18F-FDG和18F-FLT细胞结合抑制率均呈正相关（24 h：18F-FLT r=0.897，P＜0.01，18F-FDG r=0.978，P＜0.05；48 h：18F-FLT r=0.876，P＜0.01，18F-FDG r=0.910，P＜0.01）；(3)18F-FDG细胞结合抑制率与18F-FLT细胞结合抑制率呈正相关（24h：r=0.657，P＜0.01；48 h：r=0.432，P＜0.05）。结论(1)18F-FDG和18F-FLT摄取变化可以早期评价5-Fu对肺腺癌A-549细胞的化疗疗效；随着5-Fu化疗时间的延长，肺腺癌A-549细胞摄取率下降更明显；(2)18F-FDG和18F-FLT相比，18F-FDG细胞结合抑制率与18F-FLT细胞结合抑制率呈正相关，且24 h较48 h相关性高；18F-FLT早期摄取率与药物浓度的关系更密切，下降趋势更加明显，与MTT的关系更显著。因此，18F-FLT联合18F-FDG用于评价化疗疗效和指导用药将成为一种新的思路。
목적：통과측정18F-불대포도당（FDG）화18F-불대흉감（FLT）세포결합솔적변화래평고불동농도불뇨밀정（5-Fu）대폐선암A-549세포화료조기반응적료효병대량충현상제진행초보비교。방법불동농도（0.125、0.25、0.5、1.0 mg/mL）5-Fu 1.0 mL분별여폐선암A-549세포공동부육24 h、48 h，측정세포대18F-FDG화18F-FLT적세포결합변화병여MTT법측정적존활세포수목화세포억제솔비교。결과(1)5-Fu작용24 h화48 h후，18F-FDG화18F-FLT세포결합솔균수약물농도적증가이하강，량자여약물농도균정부상관（24 h：18F-FLT r=-0.950，P＜0.01，18F-FDG r=-0.844，P＜0.01；48 h：18F-FLT r=-0.939，P＜0.01，18F-FDG r=-0.786，P＜0.01）；48 h여24 h상비，량충현상제세포섭취솔균강저；(2)24 h화48 h MTT세포억제솔여18F-FDG화18F-FLT세포결합억제솔균정정상관（24 h：18F-FLT r=0.897，P＜0.01，18F-FDG r=0.978，P＜0.05；48 h：18F-FLT r=0.876，P＜0.01，18F-FDG r=0.910，P＜0.01）；(3)18F-FDG세포결합억제솔여18F-FLT세포결합억제솔정정상관（24h：r=0.657，P＜0.01；48 h：r=0.432，P＜0.05）。결론(1)18F-FDG화18F-FLT섭취변화가이조기평개5-Fu대폐선암A-549세포적화료료효；수착5-Fu화료시간적연장，폐선암A-549세포섭취솔하강경명현；(2)18F-FDG화18F-FLT상비，18F-FDG세포결합억제솔여18F-FLT세포결합억제솔정정상관，차24 h교48 h상관성고；18F-FLT조기섭취솔여약물농도적관계경밀절，하강추세경가명현，여MTT적관계경현저。인차，18F-FLT연합18F-FDG용우평개화료료효화지도용약장성위일충신적사로。
Objective To evaluate the effect of different concentrations of 5-Fu early by uptake of 18F-FDG and 18F-FLT in human lung adenocarcinoma A-549 cells. At the same time, to compare the two kinds of imaging agents preliminarily. Methods The proliferation inhibition effect by MTT and the uptake rates of 18F-FDG and 18F-FLT were detected after treatment with 5-Fu at 24 h and 48 h. Results (1)The uptake rates were negatively cor-related with the doses of 5-Fu at 24 h and 48 h after administration of 0.125, 0.25, 0.5 and 1.0 mg/mL 5-Fu (24 h:18F-FLT r=-0.950, P<0.01, 18F-FDG r=-0.844, P<0.01, 48 h: 18F-FLT r=-0.939, P<0.01, 18F-FDG r=-0.786, P<0.01) and the uptake rates of 18F-FDG and 18F-FLT with A-549 cells were lower at 48 h after administration of 5-Fu than at 24 h. (2)The binding inhibition efifciency was positively correlated with the proliferation inhibition (24 h: 18F-FLT r=0.897, P<0.01, 18F-FDG r=0.978, P<0.05, 48 h: 18F-FLT r=0.876, P<0.01, 18F-FDG r=0.910,P<0.01). (3)The binding inhibition efifciency of the two kinds of agents were correlated (24 h:r=0.657, P<0.01, 48 h:r=0.432, P<0.05). Conclusion (1)The study indicated that early therapeutic effect of 5-Fu for human lung adenocarcinoma A-549 cells could be assessed by 18F-FDG and 18F-FLT. (2)The binding efifciency of 18F-FDG was more positive correlated with 18F-FLT at 24 h than at 48 h. The relationship between early uptake rates and drug concentrations of 18F-FLT were more closely, more pronounced downward and more signiifcantly correlated with MTT. Therefore, 18F-FLT combined with 18F-FDG could be used to evaluate the curative effect of chemotherapy and medication guide which will become a new way of thinking.