中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2014年
1期
37-41
,共5页
董爽%吴晨明%胡君伟%王芹芹%简易成%何益%钟杰%熊伍军
董爽%吳晨明%鬍君偉%王芹芹%簡易成%何益%鐘傑%熊伍軍
동상%오신명%호군위%왕근근%간역성%하익%종걸%웅오군
Wnt5a%肝星状细胞%IL-1β%IL-6%肝纤维化
Wnt5a%肝星狀細胞%IL-1β%IL-6%肝纖維化
Wnt5a%간성상세포%IL-1β%IL-6%간섬유화
Wnt5a%Hepatic stellate cell%IL-1β%IL-6%Liver fibrosis
目的 研究Wnt5a对肝星状细胞(HSC)促炎细胞因子IL-1β及IL-6表达的影响,探讨Wnt5a在肝纤维化中的作用及机制.方法 采用脂多糖(LPS)和肿瘤坏死因子(TNF-α)刺激人肝星状细胞株LX-2,以qRT-PCR和Western blot检测Wnt5a表达水平变化;分别利用Wnt5a重组蛋白体外刺激LX-2和Wnt5ashRNA病毒转染LX-2,以qRT-PCR、ELISA检测促炎细胞因子IL-1β、IL-6的水平变化.结果 LPS及TNF-α可显著刺激LX-2 Wnt5a表达增加(P＜0.05);Wnt5a重组蛋白可促进LX-2细胞IL-1β、IL-6表达和分泌(P＜0.05);Wnt5a shRNA转染LX-2后IL-1β、IL-6表达水平显著降低(P＜0.05),ELISA发现Wnr5a shRNA转染组培养上清IL-1β、IL-6水平显著低于pLVX-NC-shRNA组与LX-2组(P＜0.05).结论 Wnt5a可促进HSC促炎细胞因子IL-1β、IL-6的表达而参与肝纤维化进程.
目的 研究Wnt5a對肝星狀細胞(HSC)促炎細胞因子IL-1β及IL-6錶達的影響,探討Wnt5a在肝纖維化中的作用及機製.方法 採用脂多糖(LPS)和腫瘤壞死因子(TNF-α)刺激人肝星狀細胞株LX-2,以qRT-PCR和Western blot檢測Wnt5a錶達水平變化;分彆利用Wnt5a重組蛋白體外刺激LX-2和Wnt5ashRNA病毒轉染LX-2,以qRT-PCR、ELISA檢測促炎細胞因子IL-1β、IL-6的水平變化.結果 LPS及TNF-α可顯著刺激LX-2 Wnt5a錶達增加(P＜0.05);Wnt5a重組蛋白可促進LX-2細胞IL-1β、IL-6錶達和分泌(P＜0.05);Wnt5a shRNA轉染LX-2後IL-1β、IL-6錶達水平顯著降低(P＜0.05),ELISA髮現Wnr5a shRNA轉染組培養上清IL-1β、IL-6水平顯著低于pLVX-NC-shRNA組與LX-2組(P＜0.05).結論 Wnt5a可促進HSC促炎細胞因子IL-1β、IL-6的錶達而參與肝纖維化進程.
목적 연구Wnt5a대간성상세포(HSC)촉염세포인자IL-1β급IL-6표체적영향,탐토Wnt5a재간섬유화중적작용급궤제.방법 채용지다당(LPS)화종류배사인자(TNF-α)자격인간성상세포주LX-2,이qRT-PCR화Western blot검측Wnt5a표체수평변화;분별이용Wnt5a중조단백체외자격LX-2화Wnt5ashRNA병독전염LX-2,이qRT-PCR、ELISA검측촉염세포인자IL-1β、IL-6적수평변화.결과 LPS급TNF-α가현저자격LX-2 Wnt5a표체증가(P＜0.05);Wnt5a중조단백가촉진LX-2세포IL-1β、IL-6표체화분비(P＜0.05);Wnt5a shRNA전염LX-2후IL-1β、IL-6표체수평현저강저(P＜0.05),ELISA발현Wnr5a shRNA전염조배양상청IL-1β、IL-6수평현저저우pLVX-NC-shRNA조여LX-2조(P＜0.05).결론 Wnt5a가촉진HSC촉염세포인자IL-1β、IL-6적표체이삼여간섬유화진정.
Objective To study the effects of Wnt5a on the expression of proinflammatory cytokines IL-1β and IL-6 in human hepatic stellate cells and its pathogenic mechanism in liver fibrosis.Methods The expression of Wnt5a at mRNA and protein levels in LPS-stimulated and TNF-α-stimulated human hepatic stellate cell line LX-2 were analyzed by quantitative real-time PCR and Western blot.LX-2 cells were treated with human recombinant Wnt5a protein and transfected by lentivirus carrying Wnt5a shRNA,respectively.The expression of proinflammatory cytokines IL-1 β and IL-6 in treated cells were examined by qRT-PCR and ELISA.Results LPS and TNF-α enhanced the expression of Wnt5a in LX-2 cells (P＜ 0.05).Recombinant Wnt5a protein significantly increased the expression and secretion of IL-1 β and IL-6 (P＜0.05).Compared with pLVX-NC-shRNA cells and LX-2 cells,the expression and secretion of IL-1 β and IL-6 in pLVX-Wnt5a-shRNA transfected LX-2 cells were significantly decreased (P＜0.05).Conclusion Wnt5a might participate in the progression of liver fibrosis through promoting the expression of proinflammatory cytokines IL-1 β and IL-6 in hepatic stellate cells.
