CAJ | 학술논문

目的：评估阴道炎病原生物检测试剂盒（核酸杂交法）的灵敏度和特异度．方法对400例阴道分泌物标本分别进行Amsel法、滴虫培养、真菌培养和Affirm VPIII检测方法（核酸杂交法），以Amsel法，滴虫培养，真菌培养为金标准，评价Affirm VPIII检测方法的应用价值．结果与Amsel法相比，Affirm VPIII检测法检测的灵敏度为92.2%，特异度为70.5%；与真菌培养相比，Affirm VPIII检测法检测的灵敏度为88.3%，特异度为92.9%；与滴虫培养相比， Affirm VPIII检测法检测的灵敏度为92.6%，特异度为93.8%；与金标准的一致性较好．结论与传统的检测方法相比，其具备了检测速度快，操作简捷，且一次性可同时区分和识别3种阴道炎致病病原生物，具有较高的敏感性和特异性，具有一定的临床应用价值．
목적：평고음도염병원생물검측시제합（핵산잡교법）적령민도화특이도．방법대400례음도분비물표본분별진행Amsel법、적충배양、진균배양화Affirm VPIII검측방법（핵산잡교법），이Amsel법，적충배양，진균배양위금표준，평개Affirm VPIII검측방법적응용개치．결과여Amsel법상비，Affirm VPIII검측법검측적령민도위92.2%，특이도위70.5%；여진균배양상비，Affirm VPIII검측법검측적령민도위88.3%，특이도위92.9%；여적충배양상비， Affirm VPIII검측법검측적령민도위92.6%，특이도위93.8%；여금표준적일치성교호．결론여전통적검측방법상비，기구비료검측속도쾌，조작간첩，차일차성가동시구분화식별3충음도염치병병원생물，구유교고적민감성화특이성，구유일정적림상응용개치．
Objective To assess the sensitivity and specificity of vaginitis pathogen detection reagent kit (Nucleic acid hybridization). Methods Four hundreds cases of vaginal secretion samples were detected with Amsel, vaginalis culture, fungal culture and Affirm VPIII detection method, respectively. Using the methods of Amsel, vaginalis culture and fungal culture as the gold standard, the clinical application value of Affirm VPIII detection method was evaluated. Results Compared with Amsel, the sensitivity and specificity of Affirm VPIII detection was 92.2%and 70.5%, respectively. Compared with fungal culture, the sensitivity and specificity was 88.3% and 92.9%, respectively. Compared with vaginalis culture, the sensitivity and specificity was 92.6% and 93.8%, respectively. There was a good consistency between the gold stardard and the Affirm VPIII detection. Conclusion Compared with the traditional detection methods,the Affirm VPIII detection has the advantages of fast detection speed,simple operation, and high sensitivity and specificity. In addition, it can identify three kinds of vaginitis pathogenic microorganisms at the same time,with a certain clinical value.