CAJ | 학술논문

目的：初步研究青春期一侧睾丸扭转SD大鼠性成熟后对侧睾丸的FAS/FASL系统的表达与对侧睾丸生精功能的变化情况的相互关系。方法建立青春期SD大鼠一侧睾丸扭转模型5组，A组为假手术对照组；B组为睾丸扭转组；C组为睾丸扭转+甲强龙组；D组为扭转侧睾丸切除组；E组为扭转侧睾丸切除+甲强龙组。于扭转后24h给予睾丸切除或/和注射甲强龙处理，术后1月处死，取对侧睾丸组织，HE染色检查，用免疫组织化学染色法检测睾丸生精细胞，支持细胞的FAS及FASL的表达情况，并对FAS及FASL的表达作定量分析。用放射免疫法检测血清中FSH，LH，T的值并做分析。结果 B组对侧非扭转睾丸的形态有病理性改变。各组睾丸组织同时表达FAS和FASL，B组的FAS和FASL的表达强度较其他各组有明显的升高。各组血清中FSH，LH，T的值没有统计学差别。结论青春期SD大鼠单侧睾丸扭转后对侧睾丸生精细胞发生FAS和FASL的高表达，从而导致细胞的凋亡增加，生育力下降。皮质激素可能通过抑制FAS和FASL的表达，降低生精细胞的凋亡，维持生精功能的稳定。
목적：초보연구청춘기일측고환뉴전SD대서성성숙후대측고환적FAS/FASL계통적표체여대측고환생정공능적변화정황적상호관계。방법건립청춘기SD대서일측고환뉴전모형5조，A조위가수술대조조；B조위고환뉴전조；C조위고환뉴전+갑강룡조；D조위뉴전측고환절제조；E조위뉴전측고환절제+갑강룡조。우뉴전후24h급여고환절제혹/화주사갑강룡처리，술후1월처사，취대측고환조직，HE염색검사，용면역조직화학염색법검측고환생정세포，지지세포적FAS급FASL적표체정황，병대FAS급FASL적표체작정량분석。용방사면역법검측혈청중FSH，LH，T적치병주분석。결과 B조대측비뉴전고환적형태유병이성개변。각조고환조직동시표체FAS화FASL，B조적FAS화FASL적표체강도교기타각조유명현적승고。각조혈청중FSH，LH，T적치몰유통계학차별。결론청춘기SD대서단측고환뉴전후대측고환생정세포발생FAS화FASL적고표체，종이도치세포적조망증가，생육력하강。피질격소가능통과억제FAS화FASL적표체，강저생정세포적조망，유지생정공능적은정。
Objective To investigate the relationship between the expression of FAS/FASL and spermatogenesis in the contralateral testis after unilateral testicular torsion in puberty Sprague-Dawley rats. Methods Five groups of side testicular torsion adolescence SD rats model were established as follows:group A as control group, group B for testicular torsion group,group C for testicular torsion plus methylprednisolone group, D for reverse side testes excision group, and Group E testes excision plus methylprednisolone group for reverse side. SD rats were treated with testicular resection and/or injection of methylprednisolone after torsion for 24 h. The rats were executed at postoperative 1 month, and contralateral testes were collected for histopathological examination. Expression of FAS and FASL was analyzed quantitatively by immunohistochemistry with a computer pathological image analysis system. Each rat was evaluated with regard to endocrine parameters (follicle-stimulating hormone (FSH), luteinizing hormone ( LH) and testosterone ( T) by radioimmunoassay. Results Histopathological examination of the contralateral testes showed that either orchiectomy or orchiectomy plus methylprednisolone was more successful than no treatment. Fas/FasL protein in both control group and 4 experimental groups were expressed. B group showed more expression of FAS and FASL than that of the other groups. FSH, LH and T were normal in all cases. Conclusion Unilateral testicular torsion in puberty SD rats may result in higher expression of FAS and FASL, and accelerate germ cell apoptosis and subfertility. Methylprednisolone may decrease the expression of FAS and FASL and maintain spermatogenesis in the contralateral testis after the unilateral testicular torsion.