CAJ | 학술논문

目的：采用qRT-PCR技术检测早期宫颈癌患者根治术术前外周血中CK19和SCCAg mRNA表达水平的变化，研究联合检测外周血CK19和SCCAg mRNA对早期宫颈癌患者微转移的诊断价值．方法昆明医科大学第三附属医院妇瘤科30例早期（ⅠA2～ⅡA）宫颈癌患者为实验组，15例子宫肌瘤患者和15例健康志愿者为为阴性对照组，15例晚期（ⅡB～ⅢB）宫颈癌患者为阳性对照组．收集外周血样本，qRT-PCR技术检测外周血CK19和SCCAg mRNA表达水平的变化．结果实验组外周血中CK19和SCCAg mRNA表达水平与阴性对照组、阳性对照组比较，差异有明显统计学意义（＜0.01）；30例宫颈癌患者手术前外周血CK19和SCCAg mRNA阳性检出率分别为30%（9/30）和20%（6/30），手术前联合检测二者的表达可使阳性检出率提高至43.3%，联合检测2种标志物的阳性检出率高于检测单一标志物，差异有统计学意义（＜0.05）．结论外周血CK19和SCCAg mRNA表达水平均可能作为诊断早期宫颈癌微转移的标志物．qRT-PCR技术联合检测两项指标，可提高诊断微转移的敏感度和特异性．
목적：채용qRT-PCR기술검측조기궁경암환자근치술술전외주혈중CK19화SCCAg mRNA표체수평적변화，연구연합검측외주혈CK19화SCCAg mRNA대조기궁경암환자미전이적진단개치．방법곤명의과대학제삼부속의원부류과30례조기（ⅠA2～ⅡA）궁경암환자위실험조，15례자궁기류환자화15례건강지원자위위음성대조조，15례만기（ⅡB～ⅢB）궁경암환자위양성대조조．수집외주혈양본，qRT-PCR기술검측외주혈CK19화SCCAg mRNA표체수평적변화．결과실험조외주혈중CK19화SCCAg mRNA표체수평여음성대조조、양성대조조비교，차이유명현통계학의의（＜0.01）；30례궁경암환자수술전외주혈CK19화SCCAg mRNA양성검출솔분별위30%（9/30）화20%（6/30），수술전연합검측이자적표체가사양성검출솔제고지43.3%，연합검측2충표지물적양성검출솔고우검측단일표지물，차이유통계학의의（＜0.05）．결론외주혈CK19화SCCAg mRNA표체수평균가능작위진단조기궁경암미전이적표지물．qRT-PCR기술연합검측량항지표，가제고진단미전이적민감도화특이성．
Objective Detect the change of relative expression level and positive rate of peripheral blood CK19 and SCCAg mRNA in early cervical cancer patients before receiving radical hysterectomy by qRT-PCR technique, then study the value of combined detection of peripheral blood CK19 and SCCAg mRNA expression in diagnosis of early cervical cancer micrometastasis. Methods Thirty patients with early (ⅠA2 ~ⅡA) cervical cancer from the department of gynecological tumors in the third affiliated hospital of Kunming Medical University were selected as experimental group, 15 patients with uterine myoma and 15 healthy volunteers as negative control group, and 15 advanced (ⅡB~ⅢB) cervical cancer patients as positive control group. Peripheral blood samples before receiving radical resection of experimental group and the same samples before treatment of control groups were collected. The changes of relative expression level and positive rate of peripheral blood CK19 and SCCAg mRNA were analyzed using qRT-PCR technique. Results There was significant difference in relative expression level of CK19 and SCCAg mRNA in the peripheral blood between experimental group and control groups ( <0.01) . And the positive rate of CK19 and SCCAg mRNA in the peripheral blood from 30 patients with early cervical cancer before operation in the experimental group was 30%(9/30) and 20% (6/30), respectively. Furthermore, combined detection of the expression of the two markers can improve the positive rate to 43.3%, much higher than single marker. There was also significant difference in the positive rate by combined detection of the two markers between the experimental group and control groups ( <0.05) . Conclusion CK19 and SCCAg mRNA could be used as the specific and suitable molecular markers for the detection of early cervical cancer micrometastasis. The combined detection of relative expression level and positive rate of peripheral blood CK19 and SCCAg mRNA by qRT-PCR array could significantly enhance the sensitivity and specificity for cancer cell detection.